miR-221和miR-222在急性髓细胞白血病初发患者中的表达研究

The Expression and Significance of miR-221 and miR-222 in the Acute Myeloid Leukemia.

目的探讨miR-221和miR-222在急性髓细胞白血病(AML)骨髓有核细胞中的表达及意义。方法选择16例AML初发患者和44例非白血病患者骨髓标本,分离有核细胞,抽提总RNA,以U6为内参,采用茎环Realtime RT-PCR法检测并比较AML和非白血病患者骨髓有核细胞中miR-221和miR-222的表达,同时比较这两种miRNAs在AML中M3、M4和M5三种亚型间的表达水平。结果 miR-221和miR-222在AML初诊患者骨髓中相对表达量(N=2-ΔCt)明显高于非白血病患者组(P<0.05);这两种miRNAs在M5型AML患者表达最高,但在AML三种亚型间表达水平未见显著的统计学意义(P>0.05)。结论 miR-221和miR-222有可能在为AML的诊断、治疗和预后上,提供一个新的标准和靶点指标。

Objective To investigate the expression and clinical significance of miR - 221 and miR - 222 in the marrow of acute myeloid leukemia（AML）. Methods Marrow tissues from 16 newly diagnosed AML patients and 44 non - leukemia （NL） patients were collected and mononucleated cell were separated. Total RNA from nucleated cells of these samples were extracted and analyzed for miR - 221 and miR - 222 expression by stem - loop real - time RT - PCR. The differences in subtypes M3, M4 and M5 patients and the change of miR -221 and miR -222 expression in newly diagnosed AML patients were further analyzed. Results Expression levels of miR -221 and miR - 222 were significantly higher in marrow tissues of AML patients before chemotherapy than the levels in those of non - leukemia patients （P 〈0.05）. Both miR-221 and miR-222 expression in the M5 of AML were the highest among them, but this difference in subtypes M3, M4 and M5 patients was not statistically significant（P 〉 0.05）. Conclusion miR -221 and miR -222 may play an im- portant role in the development and progression of AML and the level of miR - 221 and miR - 222 expression may be a potential target for the diagnosis, treatment and prognosis of AML.