摘要
目的观察帕罗西汀对心理应激大鼠海马5-HT及c-fos表达的影响,以揭示帕罗西汀治疗心理应激引起焦虑的中枢作用机制。方法建立大鼠心理应激模型,观察各组旷场行为和海马5-HT、c-fos的表达。结果与正常对照组比较,各应激组大鼠旷场活动明显增多,海马c-fos表达显著增加,5-HT表达减少,而帕罗西汀给药能减少应激大鼠旷场活动,下调海马c-fos表达,增加5-HT表达。结论帕罗西汀可能是通过下调海马c-fos的表达,增加了海马5-HT表达,保护了海马的功能,从而发挥了中枢抗焦虑作用。
Objective To study the effects of paroxetine on the 5 - HT and c - fos gene expression induced by psychological stress in the rat hippocampal and to explore the central mechanism of effects of paroxetine on the stress related anxiety disorders. Methods The rat psychological stress model was made by restraint stress. The behaviors of rats were tested in open - field conditions,and expression of c - fos and 5 - HT positive cells was detected by S - P immunohistochemical assay in the rat hippocampal Results The crossing scores, the rearing scores and the expression of c - los positive cells increased more significantly in the other groups than that in the control group, but decreased in the paroxetine group, the expression of 5 - HT positive cells decreased more significantly in the other groups than that in the control group, but increased in the paroxetine group. Conclusion Paroxetine may protect the function of the hippoeampus by decreasing by c - los expression and increasing 5 - HT expression in the hippocampns,which plays a central role in anxiety.
出处
《医学研究杂志》
2011年第6期114-116,共3页
Journal of Medical Research
基金
温州市科技局资助项目(Y20090198)
