抗精子抗体的定量测定及初步临床应用

Antisperm Antibody Quantitative Analysis and Preliminary Clinical Application

抗精子抗体的测定是诊断免疫学不育的重要指标之一，AsAb的类型和含量对精子的功能有较大的影响。该文就AsAb定量测定进行了研究。T－Ig、IgA和IgG的测定上限可达500U／ml；回收率在96．4％～103％之间；批内重复性＜7．0％；参考值范围为T－Ig＜74.3U／ml、IgA＜55．2U／ml和IgG＜61．U／ml。经在临床的初步应用表明，在不育患者中T－Ig超过参考值上限的比例男性23．7％，女性31．1％，但二者无统计学意义；血清中T－Ig与IgG具有较好的相关性，而与IgA有一定的相关性。可同时测定T－Ig和IgA对不育患者进行诊断。

Antisperm antibody(AsAb) detection was one of the most important index in evalution of male and fe-male infertility, concentratiln and types of AsAb affect function of sperms. With this kit, the concentrations of three Ig up to 500 U/ml can be measured; the analytical recovery of AsAb between 96. 4% to 103%; within day CV were 7. 0%,and reference value were: total Ig<74. 3 U/ml, IgA<55. 2 U/ml and IgG<61 U/ml. The result of preliminary clinical application shown:The rate which the T-Ig was more than reference upper limit were listed as below:male 23. 7%, female 31. 1%, but there is no significant. A good correlation existed between the T-Ig and the IgG,and there was some degree of correlation between the T-Ig and the IgA. The detection of T-Ig and IgA may be used as an useful means of diagnosis on in fertility.