免疫磁珠法在肾移植HLA快速分型中的应用

Application of Immunomagnetic Beads Technique for Tissue Typing in Renal Transplantation

临床为了尽快选配供受者，常常需要快速分离纯化T、B淋巴细胞，进行HLA快速分型。应用免疫磁珠法与传统方法从等待肾移植的肾脏病患者和供体捐献者的静脉或腹腔血中分离T、B淋巴细胞，进行HLA—Ⅰ、Ⅱ类抗原组织配型。结果显示，在HLA组织分型中免疫磁珠法操作简单快速，分离的淋巴细胞纯度高、活力强、实验结果准确、分型抗原易于指定，有利于临床快速选配供受体等特点。在肾移植组织配型中可取代传统的方法。

In order to match the HLA-phenotype of donor-recipient for renal transplantation rapidly and pre-cisely. Veinal blood or coeliac blood was used for separating from T、B lymphocyte by immunomagetic beads (IMB) technique and conventional means. HLA-Ⅰ、Ⅱ classes typing was performed by microlymphocytotoxicity test. The results indicated that the IMB technique was rapid and simple. It was available for obtaining highly pure and vigorous lymphocyte. The tested results were accurate and the HLA-Ⅰ、Ⅱ classes antigen were appointed easier than conventional means. It provide a rapid method to donor-recipient matching for renal transplantation. It can replace conventional means and be used widely in clinical practice.