N-糖基化位点鉴定方法和非经典N-糖基化序列

N-glycosylation sites analysis and nonconsensus N-glycosylated sequences

蛋白质的N-糖基化修饰是生物体调控蛋白质在组织和细胞中的定位、功能、活性、寿命和多样性的一种普遍的翻译后方式。N-糖基化位点是理解糖链功能的重要前提之一。应用新的糖蛋白、糖肽富集技术和质谱技术,科学家们在不同组织中完成了对N-糖基化位点的鉴定。此外,不同于经典三联子的N-糖基化序列的发现使人们对N-糖基化过程的认识向纵深发展。

N-glycosylation of proteins is the most common post-tranlational modification,strongly influencing many of their location,function,activity,life span and diversity.Our knowledge to N-glycosylation sites is one of the most important prerequisites for detailed functional understanding.Using new methods based on glycoproteins/ glycopeptides enrichment and mass spectrum techniques,scientists have been able to identify glycosylated sites in different tissues.Besides,the discovery of novel sequence patterns for N-glycosylation will help to understand the process of N-glycosylation better in future.