多药耐药鲍氏不动杆菌AmpC酶的检测及评价

Detection and evaluation of AmpC enzyme of multidrug-resistant acinetobacter baumanii

目的探讨临床分离的多药耐药鲍氏不动杆菌AmpC酶活性及基因型。方法 AmpC酶活性检测采用三维法、三联纸片法;基因检测采用PCR法。结果 20株多药耐药鲍氏不动杆菌ampC(染色体型)基因阳性14株,阳性率70.0%,ampC(质粒型)基因阳性1株,阳性率5.0%,总阳性率75.0%;三联纸片法阳性率80.0%,三维法阳性率65.0%。结论三联纸片法、三维法与基因检测结果符合率接近,三联纸片法优于经典三维法,且较基因检测法更简捷方便。

OBJECTIVE To analyze AmpC enzyme activity and its gene type of multidrug-resistant Acinetobacter baumanii isolated from the clinics. METHODS Three-dimensional test and triple combination paper disk method were used for detection of AmpC enzyme activity, and polymerase chain reaction （PCR） was used for detection of the gene type. RESULTS Among 20 multi-drug resistance A. baumanii strains, AmpC gene positive strains of chromosomal pattern were 14 stains with positive rate 70.0V0, and positive strain of plasmid pattern was 1 strain with positive rate 5. 0%. The total positive rate was 75. 0%. The positive rate was 80. 0% through triple combination paper disk method and it was 65.0% through three-dimensional test. CONCLUSION The detection results of triple combination paper disk method and three-dimensional test have almost the same coincidence rate compared with gene detection method. And triple combination paper disk method is better than traditional three- dimensional test and is more simple and convenient compared with gene detection.