用随机扩增多态性分析技术鉴定卒中相关基因连锁标记

Identification of Markers Linked to Stroke related Genes by Random \;Amplified Polymorphic DNAs Technique

目的：寻找卒中型自发性高血压大鼠卒中相关基因的遗传标记。方法和结果：用６０ 个随机引物对自发性高血压大鼠（ＳＨＲ）和卒中型自发性高血压大鼠（ＳＨＲｓｐ）两个品系基因组ＤＮＡ进行随机扩增多态性分析（Ｒａｎｄｏｍ Ａｍ ｐｌｉｆｉｅｄ Ｐｏｌｙｍ ｏｒｐｈｉｃＤＮＡｓ，ＲＡＰＤ），找到一个仅存在于ＳＨＲｓｐ 而不存在于ＳＨＲ的特异性ＤＮＡ片段，此片段长６３０ｂｐ，命名为ＳＨＲｓｐ－６３０。应用ＳＨＲｓｐ－６３０ 为探针，经Ｓｏｕｔｈｅｒｎ ｂｌｏｔ杂交证实，该序列在ＳＨＲｓｐ 为强阳性而在ＳＨＲ为弱阳性。经测序和ＧｅｎＢａｎｋ 查询，证实ＳＨＲｓｐ－６３０为新序列，ＧｅｎＢａｎｋ 序列编号为Ｇ３８０２１。结论：ＳＨＲｓｐ－６３０ 可能是与卒中相关基因紧密连锁的ＲＡＰＤ标记。

Aim:To search for the genetic markers linked to strok related genes in SHRsp. Methods:Using 60 random primers, genomic DNAs from SHR and SHRsp were analysed by RAPD (Random Amplified Polymorphic DNAs) technology. Results:One DNA fragment existing only in SHRsp, but not in SHR, was identified. This DNA fragment was 630 bp, and named SHRsp 630. Strong positive hybridization signal was found in SHRsp, and weak positive hybridization signal in SHR, by using SHRsp 630 as an hybridization probe on Southern blot. DNA sequence analysis and GenBank inquery confirmed that SHRsp 630 is a novel DNA fragment. The GenBank accession number is G38021. Conclusion:SHRsp 630 may be a RAPD marker linked to stroke related genes.