NO、AngⅡ对大鼠主动脉内皮细胞功能及形态学影响的实验研究

The Influence Reseaching of NO and AngⅡ on Endothelium Cell\; Function and Construction of Rat

目的：通过培养的大鼠主动脉内皮细胞，观察ＡｎｇⅡ对ＥＣ分泌ＮＯ，ＥＴ－１的影响及形态学变化和ＮＯ的前体物Ｌ－Ａｒｇ 对ＡｎｇⅡ的抵消作用及对ＥＣ的保护作用。结果：随ＡｎｇⅡ浓度增高ＥＣ生成ＮＯ减低，分泌ＥＴ－１ 增加，加入Ｌ－Ａｒｇ 及卡托普利后ＮＯ生成回升，ＥＴ－１ 分泌减低；高浓度ＡｎｇⅡ及ＬＤＬ胆固醇使ＥＣ释放ＬＤＨ增加，细胞收缩。适量Ｌ－Ａｒｇ 可改善之。结论：高浓度ＡｎｇⅡ，ＥＴ－１

To survey the influence of AngⅡ on secreting of nitric oxide and endothelin 1 and the changing of construction of endothelium cells, and the protecting effect of L arginine in endothelium cell. Results:Along with the AngⅡ level increasing the endothelium cell endothelin 1 production enhanced and nitric oxide decreased. High level AngⅡ or LDL cholesterol made the endothelium cell contraction and releaseing of LDH increased. Suitable L arginine could improve these changes. Conclusion: High level AngⅡ or endothelin and lower production of nitric oxide could accelerating the atherosclerosis, hypertension development.