黄连干姜药对对功能性消化不良大鼠胃排空和血清胃泌素的影响

Effects of the Drug Pair of Coptidis Rhizoma and Zingiberis Rhizoma on Gastric Emptying and Serum Gastrin in Rats with Functional Dyspepsia

目的研究黄连干姜药对的配伍规律。方法用夹尾激怒法建立功能性消化不良(functional dyspepsia,FD)大鼠模型,实验设正常组、模型组、吗丁啉组、黄连组、干姜组、药对1组(黄连-干姜=1∶3)、药对2组(黄连-干姜=1∶1)、药对3组(黄连-干姜=3∶1)、干姜大极性组和干姜挥发油组。测定各组大鼠灌胃1周后胃内色素相对残留率、小肠推进百分比和血清胃泌素的水平。结果与模型组比较,干姜大极性组和干姜挥发油组大鼠胃内色素相对残留率显著降低(P<0.05),黄连组大鼠胃内色素相对残留率显著增大(P<0.05)。黄连组、干姜组、药对1组、药对2组、药对3组、干姜大极性组和干姜挥发油组大鼠小肠推进百分比显著上升(P<0.05,P<0.01)。干姜挥发油组大鼠血清中胃泌素水平显著下降(P<0.01)。结论黄连对胃排空有显著的抑制作用,但对小肠推进有显著的促进作用。干姜不同极性部位对胃排空和小肠推进均有显著的促进作用。黄连干姜不同比例配比的药对对小肠推进均有显著的促进作用。

OBJECTIVE To study the combination laws of drug pair of rhizoma coptidis-zingiberis.METHODS Functional dyspepsia models were induced by stimulating rats on tails with forceps clip.In the experiment,wistar rats were divided into ten groups,namely,normal control group,model group,motilium group,coptidis rhizoma group,zingiberis rhizoma group,drug pair I group（coptidis rhizoma-zingiberis rhizoma=1∶3）,drug pair II group（coptidis rhizome-zingiberis rhizoma=1∶1）,drug pair III group（coptidis rhizoma-zingiberis rhizoma=3∶1）,polar constituents of zingiberis rhizoma group,essential oil of zingiberis rhizoma group,and were given orally corresponding materials.Relative residue rate of gastric pigment,percentage of intestinal propulsion and serum gastrin in all the groups were detected after 7 d treatment.RESULTS Compared with model group,relative residue rate of gastric pigment decreased significantly in polar constituents of zingiberis rhizoma group and essential oil of zingiberis rhizome group（P0.05）,relative residue rate of gastric pigment increased significantly in coptidis rhizoma group（P0.05）.Percentage of intestinal propulsion increased significantly in coptidis rhizoma group,zingiberis rhizoma group,drug pair I group,drug pair II group,drug pair III group,polar constituents of zingiberis rhizoma group and essential oil of zingiberis rhizoma group（P0.05,P0.01）.The content of serum gastin decreased significantly in essential oil of zingiberis rhizoma group（P0.01）.CONCLUSION It could be inferred that coptidis rhizoma could inhibite gastric emptying significantly,and promote intentional propulsion significantly.The different polar parts of zingiberis rhizoma could promote both gastric emptying and intentional propulsion significantly.Drug pairs of different proportion of coptidis rhizoma and zingiberis rhizoma could promote intentional propulsion significantly.