二氧化碳导致鸭梨褐变与细胞内钙的关系

Relationship between Carbon Dioxide-induced Browning and Intracellular Calcium in Yali Pears (Pyrus bretschneideri Rehd.)

为了研究CO2导致鸭梨果实褐变与细胞内钙的关系,采用高压二氧化碳(HPCD)对鸭梨进行短时处理。结果表明:经过HPCD处理后,鸭梨细胞内的水溶性钙和膜结合钙含量随处理时间延长逐渐降低;果胶酸钙和碳酸钙总含量、草酸钙含量、磷酸钙含量逐渐升高;X射线衍射结果表明碳酸钙含量有所升高;超微观察显示细胞结构已被破坏,果实发生严重褐变。因此,HPCD处理改变了鸭梨细胞内钙的存在状态和部位,使细胞质膜的透性增大,加速了果实的褐变。

Yali pears were subjected to short-time high-pressure carbon dioxide（HPCD） treatment in order to explore the relationship between their CO2-induced browning and intracellular calcium.After HPCD treatment,the content of water soluble and membrane-bound calcium decreased,whereas the contents of calcium oxalate,calcium phosphate,calcium pectate and calcium carbonate increased in Yali pears as the HPCD treatment time increased.An increase in the content of carbonate calcium was also confirmed by X-ray diffraction analysis after HPCD treatment.Moreover,the structure of brown Yali pears was destroyed,which was examined by transmission electron microscope（TEM）.Therefore,HPCD treatment can change the types and distribution of calcium in Yali pear cells,increase cell permeability,and accelerate the browning of fruits.