不同发酵条件对Fusarium.solani ZH0101产木聚糖酶的影响

Optimization of Fermentation Condition for Xylanase Production by Fusarium solani ZH0101

以本实验室筛选到的一株产木聚糖酶但不产纤维素酶的真菌Fusarium solani ZH0101为供试菌株,利用麦秸为诱导物,对产木聚糖酶的液体发酵培养基进行优化。对发酵时间、麦秸添加量、培养基起始pH值、磷酸盐、金属离子、无机氮源和有机氮源对产酶的影响进行研究,优化最佳的培养条件。优化后的液体发酵培养基条件为:麦秸20g/L、KH2PO4 4g/L、CH3COONH4 1g/L、酵母膏2g/L和起始pH值为6.0,发酵时间为14d。优化条件下所产无纤维素酶活力的木聚糖酶酶活力为26.85U/mL,能比未优化前的20.82U/mL提高近30%。

Cellulase-free xylanase has been widely applied in the food industry as well as the paper and pulp industry.Wheat straw as one of the most important agricultural by-products can be used to induce cellulase-free xylanase production through fermentation.In the present study,the optimal conditions of wheat straw fermentation by Fusarium solani ZH0101 for producing cellulase-free xylanase were 20 g/L wheat straw,4 g/L KH2PO4,1 g/L CH3COONH4,2 g/L yeast extract,initial pH of 6.0 and fermentation time of 14 days.Under the optimal fermentation conditions,the cellulase-free xylanase activity could reach up to 26.85 U/mL,which exhibited a 30% enhancement compared with the fermentation in original medium.