产α-葡萄糖苷酶抑制剂的桑树内生细菌分离及鉴定

Isolation and Identification of a Strain of α-Glucosidase Inhibitor-producing Endophytic Bacteria from Mulberry

从桑树内生细菌中筛选得到一株产α-葡萄糖苷酶抑制剂的内生细菌S-J-X-4,通过生理生化及分子生物学鉴定,初步鉴定其为萎缩芽孢杆菌(Bacillus atrophaeus)。对α-葡萄糖苷酶抑制剂的来源进行了初步研究,结果表明发酵液中的活性物质为萎缩芽孢杆菌的一种胞外分泌物。

We isolated a strain of endophytic bacteria capable of producingα-glucosidase inhibitor from mulberry and named it S-J-X-4.On the basis of physiological and biochemical characterization and 16S rDNA gene sequence,the strain was preliminarily identified to as Bacillus atrophaeus.S-J-X-4 was cultured in LB medium andα-glucosidase inhibitor was found in the fermentation broth as an extracellular product.