摘要
目的:观察白藜芦醇(resveratrol,res)对KKAy小鼠体质量和脂肪分布的影响,并探讨其中机制。方法:将36只8周龄KKAy小鼠分成3组,其中两组KKAy小鼠分别饲喂添加白藜芦醇(以饲料质量2、4g/kg添加)的AIN93G饲料,另外以12只同周龄C57BL/6J小鼠为对照。观察干预12周后白藜芦醇对小鼠体质量及皮下脂肪、附睾脂肪分布的影响;Western blot实验检测不同组小鼠附睾及皮下脂肪组织中沉默信息调节因子1(silent information regulator 1,sirt-1)、过氧化物增殖物受体γ(peroxisome proliferator-activated receptorγ,PPARγ)和CCAAT增强子结合蛋白α(CCAAT/enhancer-binding proteinα,C/EBPα)蛋白表达水平。结果:白藜芦醇干预可以在不影响摄食量的情况下减轻KKAy小鼠体质量,与模型组相比,高剂量res组小鼠体质量降低,且脂肪组织的质量及与脂肪组织系数降低;附睾及皮下脂肪组织中sirt-1的表达升高,PPARγ及C/EBPα蛋白表达水平降低。结论:白藜芦醇可以减轻肥胖小鼠体质量,降低体内脂肪含量,这可能与白藜芦醇提高脂肪组织中sirt-1的表达,从而抑制与脂肪合成及脂肪细胞分化相关蛋白有关。
Objective:To explore the effect and mechanism of resveratrol on the body weight and adipose tissue distribution of KKAy mice.Methods:Totally 36 8-week-old KKAy mice were randomly divided into 3 groups,and fed a standard AIN93G diet or a standard AIN93G diet supplemented with resveratrol at the dose of 2 or 4 g/kg.A total of 12 C57BL/6J mice fed the standard diet were chosen as the control.The body weight,subcutaneous fat and epididymal fat of the mice were measured.The expression of silent information regulator(sirt-1),peroxisome proliferator-activated receptor γ(PPARγ) and CCAAT/enhancer-binding protein α(C/EBPα) in the adipose tissue of rats were evaluated by Western blot.Results:Resveratrol could reduce the body weight of KKAy mice without dependence on food intake.Body weight and adipose tissue weight was much lower mice in the high-dose group when compared with the model group.In addition,resveratrol also enhanced the expression level of sirt-1 and inhibited the expression of PPARγ and C/EBPα in the adipose tissue of mice.Conclusion:Resveratol can ameliorate body adiposity in mice due to its promoting effect on the expression of sirt-1 in the adipose tissue and suppressive function on the expression of proteins related to lipogenesis and adipocyte differentiation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第13期289-292,共4页
Food Science
基金
广东省医学科研基金资助项目(A2010143)
关键词
白藜芦醇
肥胖
沉默信息调节因子1
过氧化物增殖物受体r
CCAAT增强子结合蛋白α
resveratrol
adiposity
silent information regulator(sirt-1)
peroxisome proliferator-activated receptor γ(PPARγ)
CCAAT/enhancer-binding protein α(C/EBPα)