白藜芦醇对KKAy小鼠体质量和脂肪分布的影响及机制

Effects and Mechanism of Resveratrol on Body weight and Adipose Tissue Distribution of KKAy Mice

目的:观察白藜芦醇(resveratrol,res)对KKAy小鼠体质量和脂肪分布的影响,并探讨其中机制。方法:将36只8周龄KKAy小鼠分成3组,其中两组KKAy小鼠分别饲喂添加白藜芦醇(以饲料质量2、4g/kg添加)的AIN93G饲料,另外以12只同周龄C57BL/6J小鼠为对照。观察干预12周后白藜芦醇对小鼠体质量及皮下脂肪、附睾脂肪分布的影响;Western blot实验检测不同组小鼠附睾及皮下脂肪组织中沉默信息调节因子1(silent information regulator 1,sirt-1)、过氧化物增殖物受体γ(peroxisome proliferator-activated receptorγ,PPARγ)和CCAAT增强子结合蛋白α(CCAAT/enhancer-binding proteinα,C/EBPα)蛋白表达水平。结果:白藜芦醇干预可以在不影响摄食量的情况下减轻KKAy小鼠体质量,与模型组相比,高剂量res组小鼠体质量降低,且脂肪组织的质量及与脂肪组织系数降低;附睾及皮下脂肪组织中sirt-1的表达升高,PPARγ及C/EBPα蛋白表达水平降低。结论:白藜芦醇可以减轻肥胖小鼠体质量,降低体内脂肪含量,这可能与白藜芦醇提高脂肪组织中sirt-1的表达,从而抑制与脂肪合成及脂肪细胞分化相关蛋白有关。

Objective：To explore the effect and mechanism of resveratrol on the body weight and adipose tissue distribution of KKAy mice.Methods：Totally 36 8-week-old KKAy mice were randomly divided into 3 groups,and fed a standard AIN93G diet or a standard AIN93G diet supplemented with resveratrol at the dose of 2 or 4 g/kg.A total of 12 C57BL/6J mice fed the standard diet were chosen as the control.The body weight,subcutaneous fat and epididymal fat of the mice were measured.The expression of silent information regulator（sirt-1）,peroxisome proliferator-activated receptor γ（PPARγ） and CCAAT/enhancer-binding protein α（C/EBPα） in the adipose tissue of rats were evaluated by Western blot.Results：Resveratrol could reduce the body weight of KKAy mice without dependence on food intake.Body weight and adipose tissue weight was much lower mice in the high-dose group when compared with the model group.In addition,resveratrol also enhanced the expression level of sirt-1 and inhibited the expression of PPARγ and C/EBPα in the adipose tissue of mice.Conclusion：Resveratol can ameliorate body adiposity in mice due to its promoting effect on the expression of sirt-1 in the adipose tissue and suppressive function on the expression of proteins related to lipogenesis and adipocyte differentiation.