沙果渣膳食纤维润肠通便作用的动物实验研究

Improving Effect of Dietary Fiber from Malusasiatica Pomace on Defecation in Mice

目的:研究发酵法制备的沙果渣膳食纤维的润肠通便功能,为开发利用沙果渣膳食纤维提供依据。方法:以小鼠为实验对象,随机分为5组,即空白对照组、便秘模型组和低(2.5g/(kg.d))、中(5.0g/(kg.d))、高(10.0g/(kg.d))3种剂量膳食纤维组。以复方地芬诺酯建立便秘模型,通过测定小肠推进率、首次排便时间、首次排黑便时间以及排便质量,判断沙果渣膳食纤维对小鼠肠道蠕动功能的影响。结果:在连续灌胃14d后,各低、中、高剂量组小鼠的墨汁推进率均高于便秘模型组,其中,中、高剂量膳食纤维组与便秘模型组间有极显著性差异(P<0.01),低剂量组与便秘模型间有显著差异(P<0.05);低、中剂量组小鼠的首次排便时间显著低于便秘模型组(P<0.05),高剂量组的首次排便时间极显著低于便秘模型组(P<0.01);各剂量组的首次排黑便时间均比便秘模型组缩短(P<0.01);中剂量组的12h的排便质量大于便秘模型(P<0.05),而高剂量组的12h的排便质量极显著高于便秘模型组(P<0.01)。结论:沙果渣膳食纤维具有良好的促进肠道蠕动和排便功能。

Objective：To explore the improving effect of dietary fiber from Malusasiatica pomace on the defecation of mice and provide the basis for the development of dietary fibers.Methods：Kunming mice were randomly divided into 5 groups including blank control group,constipation model group and DF groups at the low,middle and high doses.The effect of dietary fiber from Malusasiatica pomace on defecation function was evaluated by ink-pushing rate in small intestine,first defecation time,first black feces time and feces weight in mice with diphenoxylate-induced constipation.Results：After mice were subjected to continuous intragastric infusion for 14 days,ink-pushing rate was increased in the small intestine of mice administered with DF at low,middle and high doses as compared to the constipation model group,which exhibited an extremely significant difference between the middle or high dose groups and the constipation model group（P0.01）;however,no significant difference was observed between the low-dose group and the constipation model group（P0.05）.The first defecation time in low and middle dose groups was shorter than that in the constipation model group（P0.05）;however,the first defecation time in high dose group revealed a significant decrease when compared with that in the constipation model group（P0.01）.Compared with the model group,the first black feces time in each DF treatment group was shortened（P0.01）.The feces weight during 12 hours in the middle dose group was higher than that of the constipation model group（P0.05）;however,the feces weight in high dose group revealed an obvious increase when compared with the constipation model group（P0.01）.Conclusion：Dietary fiber from Malusasiatica pomace plays a favorable role in improving defecation and intestinal peristalsis.