阪崎肠杆菌(克罗诺杆菌属)分子检测方法研究进展

Recent Advances in Studies on Molecular Detection Methods for Enterobacter sakazakii

阪崎肠杆菌(现克罗诺杆菌属)是一种重要的食源性条件致病菌,因其能通过污染婴幼儿配方食品导致严重的新生儿脑膜炎、菌血症和小肠结肠炎等疾病,而受到广泛的关注。其分子生物学检测方法克服了传统检测方法实验操作繁琐、耗时长等问题。目前常用的分子生物学方法主要有普通PCR、实时荧光定量PCR、环介导恒温扩增、探针方法等。

Enterobacter sakazakii（Cronobacter spp.） is an emerging food-borne conditional pathogen associated with meningitis,bacteremia and necrotizing enterocolitis;especially neonatal meningitis is of high potential danger in neonates.Many bio-molecular assays like regular PCR,real-Time PCR,loop-mediated isothermal amplification,and molecular probes are highly sensitive for detecting specific food-borne pathogens,and they have overcome the disadvantages of being time-consuming,tedious operation and ambiguous results,which are usually associated with conventional detection methods.