黄芩体内H_2O_2清除系统和黄酮类活性成分积累的相关性研究

Correlation between H_2O_2 scavenging system and flavonoids accumulation of Scutellaria baicalensis

目的:研究黄芩H2O2清除系统和黄酮类活性成分积累的相关性。方法:采用HPLC方法检测黄芩悬浮细胞中黄芩苷、黄芩素的含量,采用紫外分光光度法测定总黄酮、H2O2含量和POD,PAL酶活性。结果:40℃、黑暗、PEG胁迫组总黄酮含量和PAL酶活性在12 d显著提高。NPA,NPA+40℃,40℃,NPA+黑暗,黑暗,PEG胁迫12 d前后黄芩苷呈下降趋势,黄芩素呈上升趋势,POD酶活性呈升高趋势,H2O2含量保持稳定。结论:适度的环境胁迫促进黄芩总黄酮的积累,黄芩苷在POD酶的作用下转化为黄芩素,同时维持H2O2水平的稳定以避免氧化伤害。

Objective： Study on correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis.Method： The content of baicalin and baicalein in suspension cell of S.baicalensis was determined by HPLC.The content of total flavonoids and H2O2,the activity of POD and PAL was detected by UV spectrophotometry.Result： The content of total flavonoid and the activity of PAL increased significantly in 12 days after 40 ℃,dark and PEG stress.Around 12 days after NPA,NPA＋40 ℃,40 ℃,NPA＋dark,dark and PEG stress,the content of baicalin declined and the content of baicalein rise,the activity of POD showed an increasing trend,and level of H2O2 remain stable.Conclusion： Moderate environmental stress could promote the accumulation of total flavonoids in S.baicalensis,baicalin convert to baicalein by POD,and maintaining the stability of H2O2 content to avoid oxidative damage.