当归-川芎药对及其组成药味对3种血虚模型补血作用的比较研究

Enriching blood effect comparison in three kinds of blood deficiency model after oral administration of drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb

目的:通过建立不同血虚动物模型,评价当归-川芎(归芎)药对及其组成药味的补血作用变化,探讨其配伍组对的作用特点。方法:应用乙酰苯肼溶血法、环磷酰胺化学损伤法、乙酰苯肼与环磷酰胺复合法3种方法分别复制不同的血虚小鼠模型。检测模型动物外周血常规、胸腺指数和脾脏指数、红细胞膜ATP酶活力变化。结果:3种血虚模型的以上所有指标与正常组相比均有显著变化。归芎药对及其组成药味给药后对乙酰苯肼和环磷酰胺复合血虚小鼠的大多数指标有显著影响,对乙酰苯肼溶血性血虚小鼠、环磷酰胺化学损伤性血虚小鼠的个别指标有显著影响,因此,乙酰苯肼和环磷酰胺复合血虚模型更适于归芎药对补血作用及相关机制的研究;与当归、川芎单味药相比,归芎药对配伍后在外周血指标、免疫器官及能量代谢酶的调节方面都体现出不同程度的补血作用增强趋势。结论:本研究结果为揭示当归-川芎药对相须相使组成规律提供科学依据,并有效地反应了中药生物效应多环节、多靶点的作用特点,为中药及方剂复杂物质群的生物效应研究提供借鉴,为以单味药-药对-方剂为主线的方剂配伍规律研究模式提供示范性参考。

Objective： Through establishing different blood deficiency animal model,to evaluate enriching blood effect changes of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb,and to explore the effect characteristics of their compatibility.Method： Three different methods of acetyl phenylhydrazine（APH） hemolytic method,cyclophosphamide（CTX） chemical damage method,APH-CTX complex method were used respectively to copy different blood deficiency model mice.Changes of orbit blood routine,thymus index,spleen index and ATPase activity of red cell membrane of model mice were tested.Result： Compared with normal group,all indexes had significant differences in three model mice.The drug pair and each single herb had significant impact on most indexes of the APH-CTX complex model mice,and on the individual indexes of APH hemolytic model mice and CTX chemical damage model mice.Therefore,APH and CTX complex blood deficiency model was more suitable for the enriching blood mechanism study of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma.Compared with the single herb of Angelicae Sinensis Radix and Chuanxiong Rhizoma,the drug pair of them had presented enriching blood effect at different extent with strengthening trend in regulating the invigorating blood indexes,immune organs and energy metabolic enzymes.Conclusion： The results of this research have provided scientific basis for revealing the mutual promotive composition law of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma,and responded effectively the mult-link and mult-target effect characteristics of Chinese medicine bio-effect,to offer reference for the bio-effect research of the complicated substance group of Chinese medicine and traditional Chinese medicine formulae,and to supply demonstrative reference for researching the formulae compatibility law which takes the single drug-drug pair-formulae as main line.