髓核细胞表型标记的研究进展

RESEARCH PROGRESS OF NUCLEUS PULPOSUS CELLS PHENOTYPIC MARKERS

目的综述髓核细胞表型标记的研究进展。方法广泛查阅近年关于髓核细胞表型标记的文献,并对其进行分析。结果由于不同的生物力学特性,髓核细胞和关节软骨细胞的形态及细胞外基质组分如蛋白多糖与Ⅱ型胶原α1的比率存在差异;通过髓核细胞的表面标记(CD24)、基因标记(低氧诱导因子1α、葡萄糖转运蛋白1、基质金属蛋白酶、VEGF-A等)及细胞内各种分子标记(角蛋白19和磷脂酰肌醇聚糖3、配对盒1、叉头蛋白和整联蛋白涎蛋白等)可以初步鉴别髓核细胞。结论髓核细胞与关节软骨细胞表型标记存在差异,但仍缺乏特异性标记物。

Objective To review the research progress of nucleus pulposus cells phenotypic markers. Methods The domestic and international literature about nucleus pulposus cells phenotypic markers was reviewed extensively and summarized. Results Due to different biomechanical properties, nucleus pulposus cells and articular chondrocytes have differences in morphology and extracellular components such as the ratio of aggrecan to collagen type II al. Nucleus pulposus cells can be identified by surface marker （CD24）, gene markers （hypoxia inducible factor let, glucose- transporter protein 1, matrix metalloproteinase 2, vascular endothelial growth factor A, etc）, and various markers （keratin 19 and glypican 3, paired box 1, forkhead box F1 and integrin-binding sialoprotein, etc）. Conclusion Nucleus pulposus cells and articular chondrocytes have different phenotypic markers, but nucleus pulposus cells are still lack of specific markers.