口腔鳞癌和癌前病变中端粒酶活性的检测

Study and detection of telomerase activity in oral squamous cell carcinomas and precancerous lesions

目的 探讨端粒酶活化在口腔粘膜鳞癌、癌前病变中的表达状况以及癌变过程中的作用。方法 采用端粒酶 (telomerase)聚合酶链反应 (polymerasechainreaction ,PCR)、酶联免疫吸附法和端粒重复放大 (telomererepeatamplificationprotocol ,TRAP)电泳法对 2 0例口腔粘膜鳞癌、10例口腔粘膜白斑、2 0例口腔粘膜扁平苔藓和 10例正常口腔粘膜组织中的端粒酶活性分别进行了定量和定性检测。结果 口腔鳞癌中的端粒酶活性 ( 0 12 8)显著高于癌前病变组 ( 0 0 0 5 8,0 0 0 94 )和正常组( 0 0 0 14 ) ,癌前病变组与正常组织的端粒酶活性基本一致。结论 端粒酶活化在口腔鳞癌发生、发展过程中起了重要作用 ,端粒酶可能是判断癌变潜能的良好分子生物学标记物。

Objective To find out the expression of telomerase activity in oral squamous cell carcinomas (SCC) and oral precancerous lesions Methods Telomerase activity was examined by telomerase PCR ELISA assay and telomere repeat amplification protocol (TRAP) electrophoresis 60 fresh oral specimens were selected, including 20 oral SCC, 30 oral precancerous lesions (10 oral leukoplakia, LK, 20 oral lichen planus, LP) and 10 normal mucosa (NM) Results Telomerase activity detected in SCC (0 128) was highest among all of the tissues(LK: 0 054, LP: 0 009 4, NM: 0 001 4) Statistical difference was notable between SCC and LK, LP, NM ( P <0 01) A little difference was detected between LK, LP and NM No difference was found between LK, and LP ( P >0 05) Conclusion Telomerase may play an important role during oncogenesis of oral precancerous lesions, and may be a good molecular biomarker for observing the cancerization ability of precancerous lesion and estimating its prognosis