一株假单胞菌的亚硝酸盐降解特性及其对鱼池底泥的净化作用

Nitrite degradation of a Pseudomonas sp. strain SF1 and its applicaiton in fish pond sediment

从海水养殖池的底泥中筛选到一株对亚硝酸盐有强降解作用的假单胞菌（Pseudomonas sp．）SF1，在实验室条件下研究了SF1的亚硝酸盐降解特性及其对高污染鱼池底泥的净化作用。研究结果表明，SF1具有高效降解转化高浓度亚硝酸钠的能力，其适宜降解条件为：亚硝酸钠质量浓度范围为1～1000mg／L；菌种接种量为10％；中量充气发酵；发酵时间为12～36h；发酵pH值为6．5～8．0。直接接种SF1发酵液（接种量10％，菌液A值0．36）到鱼池底泥，36h后，底泥的亚硝酸盐去除率达到67％，显示了SF1对鱼池底泥较强的净化作用。

A strain of Pseudomonas sp., named as SF1, which showed high capacity of nitrite degradation, was screened from the mud of marine culturing pond. SF1 nitrite-degrading property and its application in fish pond sediment were carried out under lab environment. SF1 showed a high ability to degrade sodium nitrite at the following conditions, 1-1000 mg/L of sodium nitrite concentrate, 10% of denitrifying bacteria inoculums, median aerating during fermentation; pH ranging between 6.5-8.0, and 12-36 h of fermentation time. Introducing SF1 fermentation broth （A value 0.36） into fish pond sediment at the ratio of 10% removed 67% of nitrite after 36 h. Our results indicate that SF1 can be used to treat fish pond sediment.