人红细胞免疫小鼠促肾上腺皮质释放激素受体的表达特征

Expression of corticotropin releasing hormone receptor on splenocyte in mice immunized with human red blood cells

目的 主要探讨人红细胞（ＨＲＢＣ） 免疫过程中脾细胞促肾上腺皮质释放激素（ＣＲＨ）受体的表达特征。方法 以ＨＲＢＣ 免疫小鼠，在动态观察抗体产生及血浆中ＣＲＨ 水平的同时，采用放射配体法检测脾细胞ＣＲＨ 受体的表达情况，并分析抗体产生及受体表达与神经内分泌肽之间的相互关系。结果 测定表明脾细胞上不仅存在ＣＲＨ 受体，并且在免疫过程中第５ 天达高峰（ Ｐ＜ ０－０５） ，相关性分析得知，其与抗体产生密切相关（ ｒ ＝ ０－９３） 。结论 ＣＲＨ 样神经肽类激素可能通过免疫细胞上的ＣＲＨ 受体进行信息传递，为进一步了解神经内分泌免疫调节机制提供依据。

Objective To explore the expression of corticotropin releasing hormone (CRH) receptor on splenocytes in mice immunized with human red blood cells. Methods The antibody titer, the level of CRH in plasma and the changes of CRH receptor expression on spleen cells from mice immunized with human red blood cell (HRBC) were tested on day 1, 3, 5, 7, 9 and 11. Results The results showed that for high affinity and low affinity the CRH receptors were expressed on spleen cells and reached the peak on day 5 ( P <0 05), during immunization with HRBC. The data suggested that there was existing close correlation between antibody and CRH receptor ( r =0.93). Conclusion It indicates that CRH like neuroendocrine peptides can transmit information by CRH receptor of lymphocytes and all this can help us know more about the mechanism neuroendocrine immunomodulation.