人体血清可见拉曼光谱与DNA紫外共振拉曼光谱的检测

Visible Raman Spectroscopy of Human Serum and Ultraviolet Resonance Raman Spectroscopy of DNA

目的 从分子水平探讨正常人和癌症患者血清中代谢产物存在差异的信息，为临床诊断癌症提供一种新的方法。方法 选用氩离子激光器输出波长为４８８－０ ｎｍ 和５１４－５ ｎｍ 的单色光，照射人体血清，检测其拉曼光谱并对实验数据进行统计分析。共计１０５２ 份血清，其中正常人血清４５２ 份；乳腺癌、子宫颈癌、膀胱癌、胰腺癌、食管癌等患者血清３３０ 份，胃癌患者血清１３８ 份；一般胃病与其他炎症患者血清１３２ 份。选用Ｎｄ∶ＹＡＧ激光器三倍频输出，泵浦染料激光器，再经倍频器获得２５０ ｎｍ波长附近的可调谐紫外光，照射核酸样品，检测其共振拉曼光谱并将实验数据与人体血清结果对照。结果 正常人血清由４８８－０ ｎｍ 波长的单色光激发的拉曼光谱的相对强度，小于由５１４－５ ｎｍ 波长的单色光激发的拉曼光谱的相对强度；癌症患者血清由４８８－０ ｎｍ 波长的单色光激发的拉曼光谱的相对强度，大于由５１４－５ ｎｍ 波长的单色光激发的拉曼光谱的相对强度；正常人与癌症患者血清拉曼光谱存在差异。人体血清的拉曼频移的平均值分别为１００６ ｃｍ －１ 、１１６１ ｃｍ －１ 和１５２６ ｃｍ － １，核酸中碱基的拉曼频移为１３３７ ｃｍ －１ 和１４８５ ｃｍ － １。

Objective Try to find out the information on differences between metabolites in serum of cancer patient and normal subject at molecular level in order to explore a new diagnostic method for cancer. Methods The human serum was excited by 488 0 nm and 514 5 nm laser light. The Raman spectra were measured from the serum of cancer patient and normal subject. The serum specimens totaled up to 1 052 consisting of 452 from normal subjects and 468 from cancer patients. The patient with inflammation is 132. Ultraviolet resonance Raman spectroscopy was used for DNA. Results There existed difference between the visible Raman spectra of serum from cancer patient and normal subject. The relative intensity of Raman spectra from normal subject excited at 514 5 nm was more pronounced than that at 480 0 nm. The relative intensity of Raman spectra from cancer patient excited at 488 0 nm was more pronounced than that at 514 5 nm. The Raman shifts of human serum were 1 006 cm -1 , 1 161 cm -1 and 1 526 cm -1 , respectively. The Raman shifts of DNA were 1 337 cm -1 and 1 485 cm -1 respectively. Conclusions The results of this study indicates that there exists difference between the metabolites in serum of cancer patient and normal subject at molecular level. The difference is not caused by nucleic acids bases in serum.