摘要
建立一种乳脂中1,3-甘油二酯(1,3-DG)定性定量的检测方法。通过薄层色谱法(TLC)对乳脂中1,3-甘油二酯进行分离和定性,再用甘油氧化酶法进行定量检测。研究结果显示:薄层色谱法能有效分离出1,3-甘油二酯;定量方法 RSD小于等于3.78%,回收率在86.73%～101.83%之间。该方法能准确、快速、灵敏地分离检测乳脂中的1,3-甘油二酯。
A qualitative and quantitative determination of the 1,3 -diacylglycerol（ 1,3 -DG） in milk fat was established. The 1,3 - DG was effectively separated and qualitatively identified by TLC , and then was quantitatively analyzed by GPO - PAP. The RSD of the method was no more than 3.78% and the recovery was 86.73% - 101.83% o The method was accurate,precise,rapid and suitable for the analysis of 1,3 -DG in milk fat.
出处
《中国油脂》
CAS
CSCD
北大核心
2011年第7期67-70,共4页
China Oils and Fats
