通塞脉微丸血清药物化学的初步研究(Ⅰ)

Serum pharmacochemistry of Tongsaimai Tiny Pill (Ⅰ)

目的研究通塞脉微丸复方大鼠给药后血清中移行成分的归属。方法建立通塞脉微丸及大鼠ig通塞脉微丸后血清的HPLC色谱分析方法;比较全方制剂、金银花、当归、甘草、玄参、黄芪和石斛各单方制剂分别给药后所得的血清样品,分析口服通塞脉微丸后大鼠血中移行成分、来源生药及其代谢产物。结果大鼠ig通塞脉微丸后从血清中发现了15种入血成分,其中10种成分为复方所含成分的原型,其中绿原酸、阿魏酸、甘草素和肉桂酸为已知确定结构的化合物,另外5种成分分别来源于金银花、当归、甘草、玄参和石斛,其具体结构还有待进一步检测分析;1种为复方给药后新产生的代谢物质;3种为给药后产生的未知化合物。结论血中移行成分及其代谢产物是通塞脉微丸的体内直接作用物质,对其深入研究将有助于阐明复方效应的物质基础。

Objective To study the adscription of transitional elements in serum of rats after ig administration of Tongsaimai Tiny Pill.Methods This study was performed by using HPLC to identify the chemical constituents of rat serum samples obtained after ig administration of the whole and individual herbs of Tongsaimai Tiny Pill.The serum samples of full preparation and the single preparations of Lonicerae Japonicae Flos,Angelicae Sinensis Radix,Glycyrrhizae Radix et Rhizoma,Scrophulariae Radix,and Dendrobii Caulis were distinguished,and the migration components of blood,source of crude drug,and its metabolites were analyzed.Results The chromatographic analysis of the rat serum obtained after ig administration of Tongsaimai Tiny Pill showed 15 compounds,10 of which were the original constituents contained in Tongsaimai Tiny Pill,and the structures of chlorogenic acid,ferulic acid,liquiritigenin,and cinnamic acid were known.Other six components were derived from Lonicerae Japonicae Flos,Angelicae Sinensis Radix,Glycyrrhizae Radix et Rhizoma,Scrophulariae Radix,and Dendrobii Caulis,and their specific structures have yet to be further analyzed;one of which was metabolite,and three were unknown compounds after ig administration of compound preparation.Conclusion The compounds absorbed into blood and their metabolites are the possible effective constituents of Tongsaimai Tiny Pill because they interact directly within the body.The results obtained in this investigation may provide useful information in the further understanding of the action mechanism of the individual and whole herbs in Tongsaimai Tiny Pill.