HPLC法测定藏药螃蟹甲中sesamoside的含量

Content Determination of Sesamoside in Tibetan Medicine Phlomis younghusbandii by HPLC

目的:建立测定藏药螃蟹甲中sesamoside含量的方法。方法:采用高效液相色谱法。色谱柱为Symmetry C18(150mm×4.6mm,5μm),流动相为乙腈-水(9∶91),流速为1.0mL·min-1,柱温为20℃,检测波长为235nm。结果:sesamoside的检测浓度在55～715μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为99.19%,RSD=1.70%(n=6)。结论:本方法简便、准确、重复性好、结果稳定,可作为螃蟹甲药材的质量控制方法。

OBJECTIVE：To establish the method for the content determination of sesamoside in Phlomis younghusbandii.ME-THODS：HPLC analysis was performed on Symmetry C18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of acetonitrile-water（9 ：91） at a flow rate of 1.0 mL·min-1.The column temperature was 20 ℃ and the detection wavelength was set at 235 nm.RESULTS：The linear ranges of sesamoside was 55～715 μg·mL-1（r=0.999 8）.The average recovery was 99.19%（RSD=1.70%,n=6）.CONCLUSION：The method is simple,accurate,good reproducibility and stable,which can be used for quality control of P.younghusbandii.