Mdy对放射性皮炎Ku70、Ku80mRNA表达的影响

Effects of Mdy Ointment on the Expression of Ku70 mRNA and Ku80 mRNA in Acute Radiation Dermatitis Rats

目的:研究本单位自制Mdy软膏对放疗所致急性放射性皮炎的作用。方法:雌性SD大鼠80只,随机分为4组,采用β射线照射大鼠,造成急性放射性皮炎,各组给药30d,观察创面情况;光镜和电镜观察创面愈合过程中组织学改变;实时荧光定量PCR法检测创面愈合过程中Ku70、Ku80mRNA表达。结果:Mdy组产生放射性皮炎的症状较轻,且愈合较模型组快;病理学检查发现Mdy组上皮细胞、纤维母细胞增值,真皮层结缔组织及胶原纤维增生,达到愈合;Mdy组对Ku70、Ku80mRNA的表达均高于模型组(P<0.05)。结论:Mdy对β射线所致急性放射性皮炎,有促进胶原蛋白合成修复创面等作用,同时上调Ku70、Ku80的表达,从而促进创面愈合。

This study aimed to evaluate the effect of Mdy ointment on acute radiation dermatitis induced by radiotherapy. Eighty female SD rats were randomly divided into four groups. The acute radiation dermatitis rat model was established through β-ray radiation. Drugs were administrated for 30 days in each group. And the appearance and healing time of wound were observed. Changes of tissue during wound healing were observed by means of light and electron microscopy. And the expression of Ku70 mRNA and Ku80 mRNA during wound healing was detected by PCR. The results showed that symptoms of radiation dermatitis were mild in the treatment group with Mdy ointment and the wound healed fast. The fibroblasts and collagen fiber were hyperplasia through the observation of light mi- croscope. The connective tissue and collagen fiber of dermis layer were hyperplasia through the observation of elec- tron microscope. The expression of Ku70 mRNA and Ku80 mRNA in the treatment group were higher than that in the model group （P 〈 0.05）. It is concluded that for acute radiation dermatitis induced by rays, Mdy ointment can promote collagen synthesis and collagen fiber proliferation. Mdy ointment upregulates the expression of KuT0 mRNA and Ku80 mRNA, which improves wound healing.