温阳益心方对心肌缺血再灌注损伤大鼠NIK／IKK／IκB／NF—κB信号转导通路的影响

Effect of Wenyang-Yixin recipe on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats

目的观察温阳益心方对大鼠心肌缺血再灌注损伤（myocardial ischemic-reperfusion injury，MFRI）NIK／IKK／IκB／NF-κB信号转导通路的影响。方法采用随机数字表法将32只SD大鼠随机分为假手术组、MI／RI模型组、温阳益心方组及氟伐他汀组。假手术组、MI／RI模型组大鼠灌服生理盐水10ml／kg·d^-1，氟伐他汀组灌服氟伐他汀悬液4mg／kg·d^-1，温阳益心方组灌服中药煎液1ml／100g·d^-1，造模前连续给药3周。可逆性冠脉左前降支结扎缺血30min再灌注2h复制MI／RI模型，应用Western blotting及RT-PCR法检测各组人鼠心肌核因子IKB诱导激酶（NIK）、IκB激酶β（IKKβ）、核转录因子抑制蛋白（IκBα）及NF-κB p65mRNA的表达。结果与MI／RI模型组比较，温阳益心方治疗组可降低心肌NIK、IKKβ、NF-κBp65mRNA水平并上调IκBα蛋白表达（P值分别为0．004、0．003、0．018、0．004，P均〈0．05）。结论温阳益心方对MI／RI大鼠有保护作用。

Objective To investigate the influence of Wenyang-Yixin recipe（WYYXR） on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats. Methods 32 SD rats were divided into 4 groups randomly, sham-operated group, model group, western medicine control group and WYYXR group. Sham-operated group and model group were given physiological saline solution （10 ml/kg）, estern medicine control group were treated with fluvastatin （4 mg/kg）, and self-made Chinese herb was perfused into rats in WYYXR group （1 ml/100 g） each time, once a day, for successive three weeks before model making. The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK, IKKβ and IκBα protein expression of myocardial tissues in rats were detected by western blotting method. And the levels of NF-κBp65mRNA were determined with RT-PCR. Results Compared with model group, the expressions of NIK, IKKβ and NF-κBp65mRNA were lower, and the protein expression of IκBα was increased in WYYXR group, there were significant differences between the two groups （P=0.004, 0.003, 0.018, 0.004, P〈0.05）. Conclusion The Wenyang Yixin Recipe can protect myocardium from MI/RI.