复方阿胶浆联合环磷酰胺对移植性小鼠lewis肺癌抑瘤率的影响

Research on the impact of compound E-Jiao slurry （CEJS） plus cyclophosphamide in the inhibition rate of lewis pulmonary carcinoma in mice

目的观察复方阿胶浆联合环磷酰胺对移植性小鼠lewis肺癌抑瘤率的影响。方法构建lewis肺癌移植瘤模型，按体重随机分为模型对照组、环磷酰胺组（CTX组）、复方阿胶浆组、复方阿胶浆大剂量联合环磷酰胺组（联合用药1组）、复方阿胶浆中剂量联合环磷酰胺组（联合用药2组）、复方阿胶浆小剂量联合环磷酰胺组（联合用药3组）共6组各10只。模型组给予生理盐水0．4ml灌胃，1次／d，共给药12次；CTX组隔日腹腔注射CTX0．2ml，共给药6次；复方阿胶浆组给予复方阿胶浆原液0．2ml、生理盐水0．2ml混合后灌胃；联合用药1组给予复方阿胶浆原液0．4ml灌胃、隔日腹腔注射CTX0．2ml：联合用药2组给予复方阿胶浆原液0．2ml加生理盐水0．2ml混合后灌胃、隔日腹腔注射CTX0．2ml；联合用药3组给予复方阿胶浆原液0．1ml加生理盐水0．3ml混合后灌胃、隔日腹腔注射CTX0．2ml。称取瘤重，按公式计算抑瘤率。结果联合用药与环磷酰胺的肿瘤抑制率均在50％以上，复方阿胶浆的肿瘤抑制率为25．88％。各实验组瘤重与模型组比较，差异有统计学意义（P〈0．05），各组瘤重按上述分窆目U顿序分别为（4．457±0．796）g、（2．105±0．651）g、（3．304±0．922）g、（1．668±0．267）g、（1．752±0．546）g、（2．075±1．061）g。复方阿胶浆不同剂量联合环磷酰胺的瘤重与环磷酰胺比较，差异无统计学意义（P〉0．05）。结论复方阿胶浆辅助化疗不能增加化疗药物对移植瘤杀伤的敏感性，但有一定的抑瘤趋势。

Objective To investigate the effects of Compound E-Jiao Slurry （CEJS） and Cyclophosphamide （CTX） on the inhibition of Lewis pulmonary carcinoma xenografts. Methods Tumor xenografts models were prepared and randomly divided into six groups by weight, including the control group, CTX, CEJS with the dosages of high, middle and low, with a total of ten mice in each group. The control mice were given normal saline 0.4ml by intragastric administration once daily, with a total of twelve times; The mice of CTX group were given CTX 0.2 ml by intraperitoneal injection every other day, with a total of six times; The mice of CEJS group were given CEJS 0.2 ml and saline 0.2 ml by intragastric administration; The mice of combination therapy group 1 were given CEJS 0.4 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day; The mice of combination therapy group 2 were given CEJS 0.2 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day; The mice of combination therapy group 3 were given CEJS 0.1 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day. The weight of tumor xenografts were measured in the experiment, the inhibition rate of tumor xenografts were calculated according to the data after the dissection. Results The combination groups and CTX alone have an inhibition rate of over 50%, and the CEJS group 25.88%. In every experimental group, the weight of tumor showed statistical significance compared with the control （P〈0.05）. The value was（4.457±0.796）g, （2.105±0.651）g, （3.304±0.922）g, （1.668±0.267）g, （1.752±0.546）g and （2.075±1.061）g respectively according to the group sequence. There was no statistical significance of tumor weight in the groups of different dosages of CEJS plus CTX compared with the CTX group （P〉0.05）. Conclusion CFAS can＇t obviously enhance the sensitivity of tumor xenografts to CTX, but there is a trend for tumor inhibition.