干扰素α-2a治疗慢性乙肝患者血清Th1/Th2亚群细胞因子的水平变化及意义

Level changing and significance of Interferon α-2a in the treatment of chronic hepatitis B subsets Th1/Th2 cytokines

目的:探讨干扰素(IFN-α)治疗慢性乙型肝炎(乙肝)抗病毒疗效与Th细胞因子的变化关系。方法:回顾性分析我院2008年4月～2010年10月收治入院的慢性乙肝患者90例的临床资料,90例的患者均采用IFN-α治疗(HBeAg阳性组),以90例健康献血员作为正常HBeAg阴性组。ELISA法检测治疗1年后患者外周血HBV-DNA及Th细胞因子IL-2、IFN-γ、IL-4、IL-10。结果:HBeAg阳性组治疗后血清IL-2、IFN-γ均值明显升高(P<0.05),HBV-DNA含量均值明显下降(P<0.05);HBeAg阳性组治疗前血清IL-2、IFN-γ与HBeAg阴性组比较均值明显升高(P<0.001),IL-4、HBV-DNA含量均值明显下降(P<0.001)。HBeAg阳性组治疗后血清IL-2、IFN-γ与HBeAg阴性组比较均值明显升高(P<0.01),IL-4、HBV-DNA含量均值明显下降(P<0.01)。结论:IFN-α治疗慢性乙型肝炎可显著抑制HBV-DNA复制;治疗后有效组免疫状态可由以Th2型为主向以Th1型为主转换。

Objective：To explore the relationship of Th cytokines and antiviral efficacy of Interferon（IFN-α） in the treatment of chronic hepatitis B（HBV）.Methods：The clinical data of 90 patients with chronic hepatitis B in our hospital from April 2008 to October 2010 were retrospectively analyzed.90 patients were treated with IFN-α（HBeAg-positive group）,90 healthy blood donors as normal HBeAg negative group.After treatment of one year,the patients with HBV-DNA and the Th cytokines IL-2,IFN-γ,IL-4,IL-10 were detected by ELISA assay.Results：HBeAg-positive group after treatment,serum IL-2,IFN-γ increased（P0.05）,HBV-DNA levels decreased（P0.05）;HBeAg positive before treatment serum IL-2,IFN-γ compared with HBeAg-negative group was significantly higher than the average（P0.001）,IL-4,HBV-DNA the mean levels significantly decreased（P0.001）.Serum HBeAg-positive group after treatment,IL-2,IFN-γ compared with HBeAg-negative group were significantly higher（P0.01）,IL-4,the mean levels of HBV-DNA significantly decreased（P0.01）.Conclusion：IFN-α treatment of chronic hepatitis B can significantly inhibit HBV-DNA replication;effective group after treatment with Th2-type immune status can be mainly oriented to the Th1-type conversion.