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非浓缩尿蛋白电泳技术在糖尿病肾病诊断中的应用

Non-Concentrated Urine Protein Electrophoresis in The Diagnosis of Diabetic Nephropathy Application
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摘要 [目的]应用SDS-AGE非浓缩尿蛋白电泳技术,分析糖尿病肾病患者蛋白尿成分特点。[方法]采用全自动琼脂糖凝胶电泳分析仪对20例正常对照组及40例糖尿病患者进行尿蛋白电泳分析。[结果]40例糖尿病患者尿蛋白经SDS-AGE电泳后,可根据蛋白质的分子量的大小顺序排列,在凝胶上可区分肾小球型、肾小管型、混合型、溢出型及生理型蛋白尿。[结论]非浓缩尿蛋白电泳分析,能早期敏感的检测尿蛋白组成成分的变化,作为判断肾脏损伤部位和程度的依据,对患者亦无任何损伤,取材方便,报告快速准确。可作为肾小球损伤诊断的特异性的实验室指标。 [Objective] SDS-AGE of non-concentrated urine protein electrophoresis,analysis of characteristics of patients with diabetic nephropathy proteinuria components.[Method] The automatic analyzer agarose gel electrophoresis in 20 normal controls and 40 cases of diabetes in patients with urine protein electrophoresis.[Results] 40 patients with diabetes mellitus urine protein electrophoresis by SDS-AGE.According to the size of the molecular weight of proteins on the gel order can be divided into glomerular type,tubular type,mixed,overflow proteinuria in type and physical type.[Conclusion] The non-concentrated urine protein electrophoresis,to early detection of urine protein sensitive to changes in the composition,when the hospital conditions,the lack of renal biopsy,can provide clinicians with diagnosis of diabetic nephropathy,kidney damage location and extent of the basis.
出处 《实用中医内科杂志》 2011年第6期86-87,共2页 Journal of Practical Traditional Chinese Internal Medicine
关键词 糖尿病肾病 非浓缩尿 蛋白电泳 Diabetic nephropathy non-concentrated urine urine protein electrophoresis
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