摘要
目的:建立HPLC法测定止嗽立效丸中吗啡的含量。方法:预处理应用固相萃取技术;液相色谱采用Shim-pack C18柱(6.0 mm×150 mm,5μm),流动相为乙腈-0.05 mol.L-1磷酸二氢钾-0.005 mol.L-1庚烷磺酸钠(10∶45∶45),流速1 mL.min-1,柱温25℃,检测波长210 nm。结果:吗啡进样量在0.026×10～2.58μg范围内线性关系良好(r=0.999 9),平均回收率为101.3%,RSD为1.2%(n=9)。结论:方法简便、准确,重复性好。
Objective： To develop an HPLC method for the determination of Morphine in Zhisou Lixiao Pills. Methods： Solid-phase extraction（SPE） was used in pretreatment. Shim-pack C18 column （6.0 mm × 150 mm, 5 μm） was used in HPLC. The mobile phase consisted of acetonitrile - 0.05 mol · L ^- 1 potassium dihydrogen phosphate solution - 0. 005 mol · L ^- 1 1-heptane sulfonic acid sodium salt solution （ 10： 45：45 ）. The flow rate was 1 mL· min^-1 , and morphine was detected by UV absorption at 210 nm. Results：The calibration curve was linear in the range of 2. 6 × 10^-2 -2. 58 μg （r =0. 999 9）. The average recovery was 101.3% , and RSD was 1.2% （n =9 ）. Conclusion：This method is simple and accurate with good repeatability.
出处
《中国药品标准》
CAS
2011年第3期181-184,共4页
Drug Standards of China
