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Morphological and genetic comparison of two strains of a Prorocentrum species isolated from Zhejiang coastal water of China and Masan Bay of Korea 被引量:3

Morphological and genetic comparison of two strains of a Prorocentrum species isolated from Zhejiang coastal water of China and Masan Bay of Korea
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摘要 In this paper, we examined the detailed morphology of two strains of Prorocentrum isolated from the coastal waters of Zhejiang (Wenling area), China, and Masan Bay of Korea. A taxonomic comparison was made among strains on the basis of morphological and molecular data. The cellular dimensions of the Chinese Wenling strain (LAMB090508) and Korean strain (PDKS0206) were similar and the cells of both strains were of asymmetric and elongated shape. The posterior end of most cells was rounded. Megacytic zones of aged cells were broader with dense tiny knobs. The roundish nucleus was located in the posterior part of the cell. A few irregular shaped chloroplasts were distributed within the cell. The nucleotide similarity of the two strains, determined from the 5.8S rDNA-ITS sequences, was 99.83%. The comparative results of morphology and molecular analysis suggest that both strains isolated from China and Korea were the identical species, Prorocentrum donghaiense Lu. In this paper, we examined the detailed morphology of two strains of Prorocentrum isolated from the coastal waters of Zhejiang (Wenling area), China, and Masan Bay of Korea. A taxonomic comparison was made among strains on the basis of morphological and molecular data. The cellular dimensions of the Chinese Wenling strain (LAMB090508) and Korean strain (PDKS0206) were similar and the cells of both strains were of asymmetric and elongated shape. The posterior end of most cells was rounded. Megacytic zones of aged cells were broader with dense tiny knobs. The roundish nucleus was located in the posterior part of the cell. A few irregular shaped chloroplasts were distributed within the cell. The nucleotide similarity of the two strains, determined from the 5.8S rDNA-ITS sequences, was 99.83%. The comparative results of morphology and molecular analysis suggest that both strains isolated from China and Korea were the identical species, Prorocentrum donghaiense Lu.
出处 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第4期832-839,共8页 中国海洋湖沼学报(英文版)
基金 Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA09Z110) the National Basic Research Program of China (973 Program) (No. 2010CB428702) Foundation of SOA (Nos. 201005031, 2009123) the Basic Research Fund of SIO (No. JG200818) Joint Project between Germany and China supported by International Bureau of BMBF at DLR (No. CHN 06/009)
关键词 PROROCENTRUM morphology 5.8S rDNA-ITS coastal waters of China Korea 东海原甲藻 沿海水域 形态学 分离株 韩国 中国 浙江 遗传
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