β-七叶皂甙钠预防术后肠粘连的实验研究

Experimental Research on Prevention of Postoperative Intestinal Adhesion by Sodium Aescinate

目的:观察β七叶皂甙钠预防术后肠粘连作用及对切口愈合的影响。方法:选用Wistar 大鼠40 只,分别刮伤盲肠浆膜,创面滴无水酒精,短暂钳夹盲肠系膜动脉,术后随机分成2 组,分别静脉注射生理盐水和β七叶皂甙钠。术后第7 d 进行大体粘连程度分级,并分别测定盲肠壁及腹壁切口组织的羟脯氨酸(OHP)水平。结果:与对照组相比,β七叶皂甙钠能明显减轻术后肠粘连程度和盲肠壁OHP水平( P<0 .01),而不影响腹壁切口OHP水平( P>0.05)。结论:β七叶皂甙钠能有效预防术后肠粘连。

To observe the effect on the prevention of postoperative intestinal adhesion and the healing of wound by sodium aescinate.Methods:Forty wistar rats were used.Adhesions were produced by scraping the cecal serosa with a scalpel blade followed by application of absolute alcohol and inducing transient ischemia by clamping the artery of the mesocecum for two minutes.Then all rats were randomized into two groups,and they were injected intravenously normal saline or sodium aescinate for 5 days respectively.On the seventh postoperative day,the severity of adhesion formation were graded,the tissue of the cecal wall and wounds of the abdominal wall were taken for determining the hydroxypoline levels.Results:Sodium Aescinate significantly decreased both the severity of adhesions and cecal hydroxyproline(OHP)levels,whereas OHP levels of wounds of the abdominal wall showed no significant differences between the two groups.Conclusion:Sodium aescinate effectively prevents postoperative adhesions.