贵阳地区汉族D12S375位点基因频率调查

A Study Of Han Population Allele Frequency of D12S375 Locus in Guiyang Area

应用ＰＣＲ技术和聚丙烯酰胺凝胶垂直电泳技术对Ｄ１２Ｓ３７５ 位点分型，在贵阳地区１０６ 位汉族个体中共检出６ 个等位基因、１８ 种基因型，基因型频率分布符合Ｈａｒｄｙ － Ｗｅｉｎｂｅｒｇ 平衡（ Ｐ＞０－０５） ，＊ １３ 频率最高，为０－４１５ １ ；＊ １６ 频率最低，为０－０１８ ８ 。观察杂合度０－７０ ，个人识别机率０－８９ ，非父排除率０－４９３ 。表明该位点是一个较好的ＤＮＡ遗传标记系统，在法医学亲子鉴定及个人识别中具有较高价值。

Using the polymerase chain reaction (PCR), D12S375 was amplified on DNA samples extracted from EDTA-blood of 106 unrelated individuals Han nationality in Guiyang. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis. 6 alleles and 18 genotypes were found at D12S375 locus, the distribution of genotypes revealed no deviation from the Hardy-weinberg equilibrium(P> 0.05). The frequency of allele 13 is the highest, which is 0.415 1,and that of allele 16 is the lowest, which is 0.018 8. The observed heterozygosity was 0 70, the power of discrimination was 0.89, and the chance of paternity exclusion was 0.493. The results of this study show that D12S375 locus is a useful DNA genetic marker system for personal indentification and paternity test in the fields of forensic science.