微量指血测定Zn Cu Ca

The determination of Zn Cu Ca in micro-blood samples

取ＥＤＴＡ抗凝指血０．１５ｍ ｌ加入１．６５ ｍ ｌ１％ 硝酸溶液中，迅速振荡混匀，放置３０ｍ ｉｎ 后再次振荡混匀，离心后取上清液，用原子吸收分光光度仪直接测定Ｚｎ、Ｃｕ。上清液稀释１０倍后测Ｃａ。此方法的平均回收率在９２．５％ ～１０３％ ，变异系数在１．４％ ～１０．４％ 。

Aanticoagulant micro-blood sample was added into 1% HNO 3 and stired immediately at Angitator.After keeping in room temperature for30 minutes.The samples were stired again,then centrifuged at a speed of 10,000 rpm for 10 minutes,the supernatant was collected to examine the concentration of Zn and Cu directly or diluted by deionied water(1:10)to detect the Ca level with atomic absorption spectrophotometry.The mean recovery rate and the coefficients of variation in this method were 92.5%～103% and 4%～10.4% respectively.