硫化氢对大鼠骨骼肌缺血再灌注所致心肌损伤的保护作用

Exogenous hydrogen sulfide prevents myocardial injury following skeletal muscle ischemia reperfusion

目的研究外源性硫化氢(H2S)对大鼠骨骼肌缺血再灌注后心肌损伤的保护作用。方法选雄性Wistar大鼠31只,随机分为4组:(1)正常对照组(8只);(2)缺血再灌注组(8只),应用止血带结扎构建大鼠双下肢缺血再灌注模型,缺血4h再灌注4h;(3)硫氢化钠(NaHS)组(8只),缺血前及再灌注前腹腔注射NaHS0.78mg/kg;(4)炔丙基甘氨酸(PPG)组(7只),缺血前及再灌注前腹腔注射PPG50mg/kg。观察各组心肌病理、血浆H2S、肌酸激酶同工酶(CK-MB)、肌钙蛋白T(TnT)及心肌胱硫醚-γ-裂解酶(CSE)活性的变化。结果与正常对照组比较,缺血再灌注组血浆H2S及心肌CSE活性明显下降,血浆CK-MB、TnT明显升高(P<0.05)。与缺血再灌注组比较,NaHS组血浆H2S浓度升高59%(P<0.05),心肌CSE活性升高93%(P<0.05),血浆CK-MB降低29%(P<0.05),血浆TnT降低67%(P<0.05);PPG组血浆H2S浓度下降20%,但无统计学差异,心肌CSE活性降低38%(P<0.05),血浆心肌酶学改变无统计学差异。心肌病理提示,缺血再灌注组心肌明显肿胀、血管充血,心肌细胞间可见明显分叶核粒细胞浸润,红细胞漏出增多,NaHS组干预后心肌损伤明显减轻。结论外源性H2S补充可以减轻骨骼肌缺血再灌注所致的心肌损伤。

Objective To explore the role of exogenous hydrogen sulfide（H2S）in the treatment of the myocardial injury following skeletal muscle ischemia reperfusion（IR）. Methods We have established the model of bilateral hindlimb IR in rats by using a tourniquet. Thirty-one Wistar rats were divided into 4 groups randomly：①Group normal（C）,②Group IR：4 hours reperfusion following 4 hours ischemia,③Group NaHS：IR＋NaHS 0.78mg/kg ip, ④ Group PPG：IR＋DL-propargylglycine（PPG）50 mg/kg ip. The pathologic changes in the myocardium were observed. The plasma levels of H2S,CK-MB,TNT and the CSE activity in myocardium in each group were measured. Results Histopathologic results indicated significant myocardial injury after hindlimb ischemia reperfusion,and it was markedly alleviated in Group NaHS. Compared with Group normal,the plasma H2S level and the myocardial CSE activity decreased obviously in Group IR（P〈0.05）,the plasma levels of CK-MB,TNT significantly increased in Group IR（P〈0.05）, Compared with Group IR,the plasma H2S level and the myocardial CSE activity were significantly higher in Group NaHS,resulting in a increase of 59% and 93% respectively. The plasma levels of CK-MB,TNT markedly decreased by 29% and 67% respectively in Group NaHS（P〈0.05）. In Group PPG,the myocardial CSE activity significantly decreased 38%,but there was no obvious difference in the plasma levels of H2S,CK-MB,TNT. Conclusions Exogenous H2S can prevent myocardial injury following skeletal muscle ischemia reperfusion.