山莨菪碱增强亚低温大鼠脑缺血神经保护作用

Enhancement in Neuronal Protection of Mild Hypothermia in Cerebral Ischemic Rats by Anisodaminum

目的:观察亚低温复合山莨菪碱颈动脉灌注对大鼠脑缺血再灌注神经元损伤的影响。方法:采用大鼠前脑缺血模型,观察海马CA1区存活神经元计数及前脑病理形态变化。结果:常温组锥体神经元大量死亡,其他前脑区域亦有明显损害;亚低温组及复合用药组存活神经元明显增多;大剂量动脉灌注组存活神经元计数显著高于其他治疗组。结论:亚低温减轻迟发性神经元坏死,大剂量山莨菪碱颈动脉灌注可增强亚低温脑保护效果,颈动脉灌注的疗效优于静脉滴注法。

Aim: To examine the effect of mild hypothermia complex anisodaminum irrigation by jugular artery on neuronal injury during cerebral ischemia and reperfusion in rat. Methods: Survival neuron count in hippocampal CA1 region and the changes of forebrain pathomorphology were investiged in forebrain ischemia model. Results: Pyramidal cells in normal temperature (NT) group died in large, there was significant injury in the other forebrain. Survival neuron in mild hypothermia and complex drug groups increased more significantly. Survival neuron in a large dose of anisodaminum irrigation by artery group was more than that in other therapeutic groups. Conclusion: Mild hypothermia could reduce delayed neuronal death. Mild hypothermia complex a large dose of anisodaminum irrigation by jugular artery could enhance the cerebral protection of mild hypothermia, and therapeutic effect of drug administrated by jugular artery excelled over that did intravenously.