HL-60细胞内一种新的全反式维甲酸应答基因的克隆

Cloning of a new cDNA: Responded to all trans retinoic acid in HL 60 cell differentiation

利用差示PCR 技术从HL60 细胞内克隆出一个新的、差异表达的cDNA 片段,并将其命名为W1 基因。Northern 印迹杂交证实,用全反式维甲酸(ATRA) 诱导HL60 细胞分化时,W1 基因的转录水平在诱导早期增加而在诱导24 h 后关闭。推测W1 基因可能在HL60

In this paper a new type of cDNA fragment named as W 1 gene was first cloned and sequenced from ATRA induced HL 60 cells by using differential display PCR (DD PCR) and DNA sequencing techniques. These differentially expressing products of the gene responding to ATRA were further confirmed by Northern blotting analysis. The results showed that the expressing level of this gene induced by ATRA (10 -6 ?mol·L -1 ) for 16?hrs was much higher than that in the control HL 60 cells, but its expression in HL 60 was reduced to an unestimable level after induction of ATRA (10 -6 ?mol·L -1 ) for 24?hrs. It suggests that ATRA may increase the expression of W 1 gene during the early stage of HL 60 cell differentiation. The exact mechanism of action is being studied further.