福尔马林固定组织电镜标本制作法

Sample preparation for electron microscope with tissues fixed with formalin and embeded in paraffin

本文介绍了一种能使福尔马林固定、石蜡包埋的组织达到电镜观察要求的样品制备方法。对于取材新鲜、固定及时的石蜡包埋组织,脱蜡后再以4 % 戊二醛固定24 h ,1 % 锇酸后固定1 ～2 h ,再常规丙酮脱水、Epon812 包埋、超薄切片,可获得基本上符合诊断要求的超微结构。此外。

A method of sample preparation for electron microscope is introduced. After paraffin embedded tissues, fresh and fixed on time,were deparaffined, the tissue were fixed in 4% glutaraldehyde for 24 hours, in 1% osmic acid for 1～2 hours, and dehydrated with acetone and embedded in Epon812; then they were cut into ultrathin sections. By this method, better ultrastructure of paraffin embedded tissues was obtained, and the method could satisfy the demand for observation under electron microscope. In addition, some important proceedings were also discussed in the paper.