浅析凝血实验检测的影响因素

Analyses the Influence of Lab Testing Blood Coagulation Factor

目的探讨凝血试验检测的影响因素,以期提高检测结果的准确性。方法抽取我院80例体检者的血液标本,分别置于三支试管中,分别在取血完毕,4、8、12、24h分离血清,采用ETAGO血凝分析及配套试剂分别对在室温(20～28℃)进行凝血试验,测得凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)及凝血酶时问(TT),并将这3个时间段测得的指标进行比较,结合相关文献及临床实际对凝血试验检测的影响因素进行分析。结果 APTT在4h内,PT在8h内测定的结果与即刻测得的结果无明显差异(P>0.05)。而FIB、TT在24h内检测结果无明显差异(P>0.05)。结论凝血试验检测的影响因素有很多,但时间是一个不可忽视的方面,标本需在4h内完成检测,以确保检测结果的准确性。

Objective To investigate the influence of blood clots,test,in the hope of improving detection results factors of accuracy.Methods 80 cases of our medical extract blood samples in three teams respectively,respectively,in a test tube in take blood finished,4,8,12,24h separation,serum,use,ETAGO blood clots and supporting the reagent for analyzing respec-tively in room temperature （20-28℃） clotting test,measurement prothrombin time （PT）,activate part blood coagulation time （APTT） live enzymes,fibrinogen （FIB） and thrombin asked （TT）,and will these three times measurement indicators for comparison,in conjunction with the relevant literature and clinical practice of clotting factors affecting the test are analyzed.Results In 4h APTT within 8h in determination of PT and the results in immediate measurement results have no obvious difference （P〉0.05）.But within 24h FIB,TT within test results have no obvious difference （P〉0.05）.Conclusion Clotting factors affecting the test,there are many,but time is an unavoidable aspect,specimens in finish testing,presents to ensure the accuracy of the test results.