肝门部胆管癌组织内血管形成与细胞凋亡相关性的研究

Correlation between angiogenesis and apoptosis in hilar cholangiocarcinoma

目的 研究肝门部胆管癌中细胞凋亡和血管形成的相互关系。方法 采用原位末端标记技术和抗因子Ⅷ的抗体分别检测肝门部胆管癌细胞凋亡指数和瘤内微血管的密度，同时用免疫组化法检测血管内皮细胞生长因子（ＶＥＧＦ） 和胆管癌细胞凋亡调控基因ｂｃｌ２ 的表达。结果 肝门部胆管癌组织ＶＥＧＦ阳性表达率５５－５６％ （２０／３６），ＶＥＧＦ阳性表达与ＭＶＤ呈正相关（Ｐ＜ ０－０１） 。高血管组的ｂｃｌ２ 阳性表达明显高于低血管组（ Ｐ＜ ０－０５），而低血管组的细胞凋亡指数高于高血管组（ Ｐ＜０－０１）。ｂｃｌ２ 表达丰富的区域，ＶＥＧＦ的表达率高。结论 肝门部胆管癌血管形成影响细胞凋亡的敏感性和发生率。

Objective To explore the relationship between occurrence of apoptosis and angiogenesis in hilar cholangiocarcinoma. Methods Thirty six surgical specimens from patients with hilar bile ducts carcinoma were studied by immunohistochemical stainings for vascular endothelial growth factor (VEGF), and von Willebrand factor as quantitative markers for microvessel density. We also determined apoptotic index (AI) using in situ end labeling (ISEL) and expression of bcl 2, an important apoptosis related gene in hilar cholangiocarcinoma. Results The positive expression rate of VEGF was 55.6%(20/36) in hilar cholangiocarcinomous tissue. There was positive correlation between expression of VEGF and MVD (P<0.01). In the group with high vessel density the positive expression rate of bcl 2 was significantly higher than that of the group with low vessel density (P<0.05), whereas AI was higher in the latter group than in the former (P<0.01). In regions with rich expression of bcl 2, the expression rate of VEGF was high. Conclusion Hilar cholangiocarcinoma angiogenesis may contribute to reduction of apoptosis in tumor cells.