摘要
目的 阐示胰腺良恶性疾患细胞凋亡的存在形式及凋亡介导基因ＩＣＥ在胰腺良恶性疾患中的表达水平。方法 由凋亡原位分析、免疫组化及Ｗｅｓｔｅｒｎ 印迹杂交，对３８ 例胰腺癌石蜡标本、２１ 例胰腺癌冰冻标本、２０ 例慢性胰腺炎冰冻标本进行凋亡及ＩＣＥ表达的半定量统计学分析。结果 肿瘤细胞凋亡原位分析结果为１－５５±０－８９，同组织非肿瘤细胞为２－３４±０－７１，二者差异有极显著意义（Ｐ＜０－０１） ；免疫组化示ＩＣＥ在胰腺癌组织中的表达为１－３３±０－７９，慢性胰腺炎组织为２－６０ ±０－８１，二者差异有极显著意义（Ｐ＜０－０１）；Ｗｅｓｔｅｒｎ 印迹示ＩＣＥ前体Ｐ４５ 及中间聚合体Ｐ３０ 在胰腺癌组织中的阳性率为９／２１（４２－９％ ），慢性胰腺炎组织为１６／２０（８０ ％） ，二者差异有极显著意义（Ｐ＜０－０１）。结论 肿瘤细胞凋亡状态的减弱及ＩＣＥ在胰腺良恶性疾患间的表达差异提示正常状态凋亡机制的被抑制是细胞癌变的可能途径。
Object The presence of apoptosis and the expression of ICE, a kind of apoptosis mediating gene, in tissues of human pancreatic carcinoma and chronic pancreatitis were investigated. Method Thirty eight paraffin embedded tissues of pancreatic carcinoma were used for in situ apoptosis assay, and 21 frozen tissues of pancreatic carcinoma and 20 frozen tissues of chronic pancreatitis were used for both immunohistochemistry and Western blot analyses. In addition, the staining intensities observed from in situ apoptosis assay and immunohistochemistry were scored semiquantitatively. Results With regard to in situ apoptosis assay, the staining score for tumor cells (1.55±0.89) was significantly lower than that of benign cells (2.34±0.71) within the same sections (P<0.01). Similarly, the immunohistochemistry staining with the antibody against ICE in the carcinoma tissues showed significant difference in comparison with that in the chronic pancreatitis tissues (1.33±0.79 vs 2.60±0.81, P<0.01). Western blot analysis revealed that the positive bands migrating at Mr. 45?000 and Mr. 30?000 were detected in 9/21 (42.9%) of the carcinoma tissues, whereas in 16/20(80%) of the chronic pancreatic tissues. There was also significant difference (P<0.01) between the two groups. Conclusion The suppressed apoptotic state of the tumor cells and the expression difference of ICE in malignant and benign pancreatic tissues indicate that the inhibition of normal apoptosis is a possible way for carcinogenesis.
出处
《中华肝胆外科杂志》
CAS
CSCD
1999年第6期381-384,共4页
Chinese Journal of Hepatobiliary Surgery
