前列腺组织端粒酶活性检测对前列腺癌的诊断价值

Detection of telomerase activity in prostate needle biopsy samples

目的 通过检测前列腺穿刺组织端粒酶活性 ,探讨其在前列腺癌诊断和预后中的临床价值。 方法 2 0例前列腺癌标本和 16例癌旁组织均来自前列腺穿刺活检 ,14例良性前列腺增生(BPH)标本取自前列腺摘除手术 ,均经病理证实。采用改良的TRAP 银染方法检测端粒酶活性 ,并进行半定量分析。 结果 2 0例前列腺癌标本中 18例测得端粒酶活性。 16例前列腺癌旁组织中 ,7例前列腺上皮内瘤 (PIN) ,2例癌旁BPH组织有端粒酶活性表达。 14例非癌患者的良性前列腺增生标本端粒酶活性均为阴性。前列腺癌端粒酶活性表达与肿瘤分级、分期无明显相关性 ,但与血清前列腺特异抗原水平之间呈正相关。 结论 前列腺穿刺活检组织端粒酶活性检测可作为前列腺癌诊断的一项新的敏感指标 ,其在前列腺癌预后中的价值有待于进一步的研究。

Objective To detect telomerase activity inprostate needle biopsy samples and its role in diagnosis and prognosis of prostate cancer. Methods 20 tissue samples of prostate cancer tissues and 16 samples of adjacent tissues were obtained by needle biopsy guided by transrectal B ultrasonography. 16 samples of benign prostatic hyperplasia tissues were obtained by prostatectomy and were confirmed pathologically. Telomerase activity was revealed by modified PCR based telomeric repeat amplication protocol (TRAP) silver staining assay. Results Telomerase activity was revealed in 18 cases of prostate cancer. In tissues adjacent to prostate cancer, telomerase activity was found in 7 of 11 cases of prostatic intraepithelial neoplasia (PIN), and in 2 of 5 cases of BPH. None of tissue samples from patients with BPH showed telomerase activity. Conclusions Telomerase activity in prostate needle biopsy samples might be an useful marker for detecting prostate cancer and biological malignancy.