骨形成蛋白-2在卵巢癌组织中的表达检测及其对卵巢癌细胞增殖影响的研究

The research and test of bone morphogenetic protein-2 in ovarian cancer tissue and its effect on ovarian cancer cell

目的：探讨骨形成蛋白-2（BMP-2）在卵巢癌上皮性癌（EOC）中的表达检测及对卵巢癌细胞增殖影响的研究。方法：选择该院2007年4月~2010年7月收治的98例卵巢癌患者,提取患者肿瘤细胞后,采用SABC免疫组化方法和RT-PCR技术,检测卵巢上皮性肿瘤和正常卵巢组织中BMP-2蛋白及其mRNA的表达,分析其与卵巢癌细胞增殖及癌细胞组织分级之间的关系。结果：BMP-2蛋白在卵巢癌细胞组织中的阳性表达率为79.59%（78/98）,与正常卵巢组织中的阳性表达率30.43%（7/23）比较有统计学差异（P〈0.01）;BMP-2蛋白与mRNA在卵巢癌组织高级别组（Ⅲ级、Ⅳ级）中的表达显著性高于低级别组（Ⅰ级,Ⅱ级）,表明了随着癌细胞级别的升高,BMP-2的表达也逐渐增强。结论：BMP-2的表达对卵巢上皮性癌细胞增殖具有抑制的作用,同时BMP-2蛋白及其mRNA的异常表达可能与卵巢癌的恶性程度相关,因此可以作为检测肿瘤的一个标志物在临床中应用。

Objective：To investigate the expression of BMP-2 protein in epithelial ovarian cancer（EOC） tissue testing and proliferation of ovarian cancer research.Methods：From April 2007 to July 2010,98 patients with ovarian cancer in this hospital were enrolled.The tumor cells were extracted by SABC immunohistochemistry and RT-PCR technique to detect ovarian cancer and normal ovarian tissue BMP-2 protein and mRNA expression analysis in ovarian cancer cell proliferation and the relationship between cancer tissue classification.Results：BMP-2 protein in ovarian cancer cells,the positive expression rate was 79.59%（78/98）,and normal ovarian tissue,the positive expression rate of 30.43%（7/23） significant difference（P0.01）;BMP-2 protein and mRNA in ovarian cancer with high-level group（Ⅲ,Ⅳ level） expression significantly higher than those of the low-level group（Ⅰ,Ⅱ grade）,indicating that cancer cells with elevated levels,BMP-2 expression also gradually increased.Conclusion：BMP-2 expression in ovarian cancer cell proliferation inhibitory effect,BMP-2 protein and mRNA may be associated with abnormal expression of the degree of malignancy of ovarian cancer related,it can be used as a marker of tumor detection in clinical application.