FHIT和HIF-1α在子宫内膜异位症中的表达及意义

Expression of FHIT and HIF-1α in endometriosis

目的：探讨脆性组氨酸三联体（FHIT）和缺氧诱导因子（HIF-1α）在子宫内膜异位症中的表达。方法：应用免疫组化SP法检测30例子宫内膜异位症异位内膜和在位内膜及30例同期单纯子宫肌瘤患者子宫内膜（对照组）中FHIT和HIF-1α的蛋白的表达。结果：FHIT在正常子宫内膜、在位子宫内膜、异位子宫内膜中的阳性表达率逐渐降低（χ2=28.865,P〈0.000 1）,在内异症r-AFS分期中比较,在位子宫内膜FHIT的表达差异无统计学意义（χ2=2.337,P=0.341）;而异位内膜中的FHIT表达差异有统计学意义,Ⅰ~Ⅱ期高于Ⅲ~Ⅳ期（χ2=6.816,P=0.019）。HIF-1α在正常子宫内膜、在位子宫内膜、异位子宫内膜中的阳性表达率逐渐升高（χ2=55.798,P=0.000）,在内异症r-AFS分期中比较,HIF-1α在在位内膜和异位内膜的表达差异均无统计学意义（χ2=0.867,P=1.000;χ2=2.049,P=0.583）;FHIT与HIF-1α在内异症中表达有相关性,FHIT与HIF-1α的表达水平在在位内膜中呈显著负相关（r=-0.443,P=0.014）;FHIT与HIF-1α的表达水平在异位内膜中呈显著负相关（r=-0.462,P=0.010）。结论：FHIT与HIF-1α可能共同促进了内异症的发生、发展。

Objective：To detect the expression of FHIT and HIF-1α in endometriosis（EMT）,so as to investigate the pathogenesis of EMT.Methods：Immunohistochemistry was used to examine the expression of FHIT and HIF-1α in the eutopic and ectopic endometrium of 30 patients with endometriosis and in the endometria in 30 patients with hysteromyoma.Results：The rate of FHIT expression in the normal endometrium,eutopic endometrium and ectopic endometrium were gradually reduced.The difference of the FHIT expression in each groups was statistically significant（χ2=28.865,P0.001）..FHIT of EMT in eutopic endometrium showed no difference in difference r-AFS stages（χ2=2.337,P=0.341）;FHIT of EMT in ectopic endometrium showed significant difference in difference r-AFS stages;FHIT in Ⅰ～Ⅱ stage was higher than that of Ⅲ～Ⅳ stage（χ2=6.816,P=0.019）.The difference of the FHIT expression in ectopic endometrium was statistically significant.The rate of HIF-1α expression in the normal endometrium,eutopic endometrium and ectopic endometrium were gradually rise.The difference of the HIF-1α expression in each groups was statistically significant（χ2=55.798,P=0.000）.HIF-1α of EMT in eutopic endometrium and in ectopic endometrium showed no difference in difference r-AFS stages.（χ2=0.867,P=1.000;χ2=2.049,P=0.583）.There was a significant minus correlation between the expression of FHIT and HIF-1α in endometriosis.There was a significant minus correlation expression in the eutopic endometrium（r=-0.443,P=0.014）;There was a significant minus correlation expression in the ectopic endometrium（r=-0.462,P =0.010）.Conclusion：FHIT and HIF-1α may have a synergetic action in the beginning and developing of endometriosis.