黄芪注射液对镉致孕鼠毒性的拮抗作用

Antagonism of Radix Astragali Mongolici injection on chromium induced toxicity of pregnant mouse

目的:探讨黄芪注射液对镉致孕鼠毒性的拮抗作用。方法:将受孕Sprague Dawley(SD)大鼠随机分成3组:黄芪注射液+氯化镉组(A组)、氯化镉组(Cd组)和对照组(C组)。A、Cd组分别每天饮用含40 mg/L氯化镉的水,C组饮用等量蒸馏水;A组每天腹腔注射黄芪注射液10 g/kg/d,Cd组和C组每天腹腔注射等量蒸馏水;各组每天均喂食普通饲料。20天的孕鼠麻醉后心脏取血,测定血清镉(Cd)、谷胱甘肽过氧化物酶(glutathiOne Pe roxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutas,SOD)和相关性激素(雌激素E2孕激素P)含量,并取胎盘组织冷冻保存测其中镉含量。结果:与Cd组(0.68±0.12、0.23±0.04、3.45±0.68、1.78±0.09、11.73±0.68、22.97±1.07)相比,A组孕鼠血清镉(0.23±0.04)、胎盘镉含量(0.12±0.03)下降(P<0.05),血清中GSH-Px、SOD、E2、P含量(12.57±2.10、7.47±1.35、18.36±0.96、40.75±1.27)升高(P<0.05)。结论:黄芪注射液可拮抗镉对孕鼠的毒性,作用机制可能与其有效成分清除自由基和抗氧化作用等有关。

Objective：To detect the antitoxicity function of Radix Astragali Mongolici injection for cadmium to pregnant mouse.Methods：The pregnant mouse were randomly divided into three groups： Astragali Mongolici injection and cadmium chloride group（A group）,cadmium chloride group（Cd group） and control group,the mouse of A and Cd groups drank cadmium chloride solution respectively,C group took the same volume vapor water,A group were injected Astragali Mongolici 10 kg/d,as control Cd and C groups were given the same dose as A group.Every group was feed the general food to take the twenty days gestational aged mouses blood to test the level of serum Cd,GS-Px,SOD,E2,P and the content of Cd in placenta.Results：Compared with Cd group,the content of A group＇s serum and placental Cd decreased;while the level of GSH-Px,SOD,E2 and P were increased.Conclusion：Astragali Mongolici injection has anti-toxicity of Cd to pregnant mouse,may correlate with eliminating the free radical and anti oxygenesis.