骨肉瘤肺转移患者血浆中整合素α_2β_1的研究

A study on the integrin α_2β_1 in plasma of patients with osteosarcoma lung metastases

目的整合素α_2β_1为整合素家族的一员,近来的研究发现整合素在肿瘤的转移发展中有重要的作用。本文通过对骨肉瘤肺转移患者血浆中整合素α_2β_1表达的研究,探讨其与肺转移发生的相关性及临床意义。方法选取肺转移的骨肉瘤16例,未转移的骨肉瘤20例,普通体检者32例,采集血样离心处理后取血浆,用ELISA法检测整合素α_2β_1。结果正常体检者(1.85±0.39)μg/L,骨肉瘤未转移者(2.06±0.41)μg/L,骨肉瘤肺转移者(3.15±0.80)μg/L。结论实验结果表明骨肉瘤肺转移患者血中整合素α_2β_1表达水平较正常人和未转移骨肉瘤患者是明显升高的。这对于高度恶性的骨肉瘤的诊断及骨肉瘤患者的预后的判定可能有帮助,整合素α_2β_1作为药物治疗的靶分子的可能,及其对抗物能否起到抑制骨肉瘤转移传播有待于进一步研究。

Objective Integrin α2β1 is a member of integrin family. Recently, integrin is found to play an important role in the metastasis during the development of tumor. Based on the study of the pulmonary metastasis of osteosarcoma in patients with integrin α2β1 expression, the relevance and clinical significance of integrin α2β1 in lung metastasis was invastigated. Methods 16 cases of osteosarcoma with lung metastases, 20 cases of osteosarcoma without metastasis and 32 patients underwent normal physical examination were included. Plasma samples were obtained after blood samples were processed by centrifugation and were determined by Enzyme-linked immunosorbent assay （ELISA） to detect integrin α2β1. Results Regarding integrin α2β1, the results of patients underwent normal physical examination was （1.85 ±0.39）ug/L, patients of osteosarcoma without metastasis was （2.06±0.41）ug/L, and patients of osteosarcoma with lung metastasis was （3.15±0.80）ug/L respectively. Conclusions Results showed that there was an obvious rising in the expression level of integrin α2β1 in patients of osteosarcoma with lung metastasis compared with those of normal volunteers and osteosarcoma patients without metastasis. It may be helpful for the diagnosis of this highly malignant osteosarcoma and the determination of prognosis for patients with osteosarcoma. The possibility of Integrin α2β1 being a potential target molecules of medication, and whether its antagonists can protect against metastasis of osteosarcoma remain to be further investigated.