人直肠癌淋巴管超微结构的特征

Morphologic features of abnormal ultrastructure of the lymphatic vessel in human rectal cancer

目的观察人直肠癌淋巴管的超微结构特征,为进一步探讨癌组织的淋巴道转移机制提供形态学依据。方法取临床直肠癌患者手术切除的组织,按癌组织中央区、周边区及正常区分别取材,应用超薄切片电镜观察对直肠癌组织内淋巴管的形态结构进行研究。结果在直肠癌组织中心区未见淋巴管;而癌组织周围区淋巴管的密度较癌组织及正常区增高,且管径较大,形态不一;淋巴管内皮细胞膜及细胞器有明显变化,内皮细胞间连接开放增多,常见淋巴管管壁的破坏和不完整;其中观察到两种功能状态不同的淋巴管,一种线粒体丰富,囊泡多,功能旺盛,另一种这些结构有缺陷。结论癌周淋巴管存在两种功能状态的淋巴管,直肠癌很可能通过侵犯功能旺盛或代偿的淋巴管,破坏溶解管壁和开放连接而引起淋巴道转移。

Objective To observe the abnormal ultrastructure of the lymphatic vessels in human rectal cancer in order to provide the morphology theory basis for mechanism of the lymphatic vessels invasion in cancer tissue.Methods By means of resinembedded ultrathin section,the samples were cut in the center,periphery and normal regions of the postoperative tissue of the rectal cancer respectively for observation of the lymphatic structures by transmission electron microscopy.Results No lymphatic vessel was found in the central area of the rectal cancer tissue,but many lymphatic vessels were found in the periphery region.The morphology of these lymphatic vessels were inconsistent with each other with the cavity dilated,and the changes of the lymphatic endothelium and organelles occurred obviously,there were two different sorts of lymphatic vessels,one was functional with plenty mitochondria and vesicles,the other was structural defect.Conclusion There are two different kinds of lymphatic vessels.The rectal cancer cells metastasis along the lymphatics and enter lymphatic lumen through the endothelial dissolution and the opening of endothelial junctions of functional lymphatic vessels.