检测中草药提取物中黄曲霉毒素的高灵敏方法

High sensitive method for the detection of aflatoxins in extract from Chinese medicinal herb

目的建立中草药提取物中黄曲霉毒素B1,B2,G1,G2的高灵敏测定方法。方法样品经提取和过滤后用免疫亲和柱净化和衍生,然后采用不同比例（15%~30%）乙腈/水梯度洗脱,用具荧光检测器的高效液相色谱仪检测。结果检出限：黄曲霉素G1为0.09 ppb;B2为0.02 ppb;B1为0.03 ppb;G2为0.04 ppb,均低于文献报道的检出限,样品加标回收率为90%~103.2%。结论该方法简单、快速、准确、灵敏度高,可用于中药中黄曲霉毒素含量的测定。

Objective To establish a rapid method of high performance liquid chromatography（HPLC） plus pre-column derivatization for the determination of aflatoxins（AFT） in Chinese medicine herbal extract.Methods The Chinese medicinal herbs were extracted with methanol and the extract was cleaned up with a solid phase extraction column.The analytes were eluted on C18 column with different proportion（15%-30%） acetonitrile,water gradient elution.Results The spiked recoveries were 90%-103.2%.The AFT limits of detection were 0.09 ppb（G1）,0.02 ppb（B2）,0.03 ppb（B1）,0.04 ppb（G2）.The results were lower than those of the previous study.Conclusion The modified method was validated by the analysis of a certified reference material and shown to be useful for the determination of aflatoxins in herbal samples of high acidity.