灵芝制剂治疗猴获得性免疫缺陷综合征的疗效观察

Effectiveness of Gonderma Lucidum Preparation in Treating Simian Acquired Immune Deficiency Syndrome

目的初步观察灵芝制剂治疗猴获得性免疫缺陷综合征(SAIDS)的疗效。方法以5只成年雌性中国恒河猴为研究对象,经直肠感染猴免疫缺陷病毒(SIV)SIVmac239株,17个月后经实验室检查证实SAIDS造模成功。给予其中3只每日口服1次灵芝制剂,共8个月,整个实验时间为1年。对照组2只猴不服药。结果治疗组猴#393,对照猴#361、#348分别死于实验开始后3.5、6、11个月,尸检显示为艾滋病后期和机会性感染,治疗组猴#374和#381存活至实验结束。治疗组猴#374的外周血CD4+T淋巴细胞在治疗后6个月较治疗前上升30%,其后有些波动。治疗组猴的血浆病毒载量在治疗过程中逐渐下降达1 log10,对照组猴的病毒载量没有下降。病理检查结果显示,治疗组猴出现淋巴组织免疫重建、胸腺再生、海马回神经细胞新生,脑垂体、松果体、甲状腺、肾上腺和卵巢等内分泌器官在形态学上均恢复趋向常态;对照组猴则表现为淋巴结耗竭、脾脏萎缩、胸腺消失,内分泌噐官处于高-中度萎缩,甚至衰竭状态。结论灵芝制剂可能对SAIDS猴的免疫、神经和内分泌系统具有一定的保护作用。

Objective To explore the effectiveness of the traditional Chinese herbal medicine Lingzhi（Gonoderma lucidum） preparation in treating simian acquired immune deficiency syndrome（SAIDS）.Methods Five female adult Chinese rhesus monkeys were inoculated rectally with SIVmac239,and were all diagnosed as SAIDS by laboratory and clinical examinations 17 months later.Of these 5 monkeys,3（#393,#374,and #381;treatment group） were orally administered with Gonoderma lucidum（2 spores powder capsules plus 2 spores oil capsules on a daily basis）,and the remaining other two monkeys（#348 and #361） served as control and did not receive treatment.Results Animal #393（treatment group）,#361（control group） and #348（control group） died of SAIDS（opportunity infection） 3.5 months,6 months,and 11 months later,respectively.Two animals（#374 and #381） survived.The necropsy revealed depletion and/or exhaustions of their lymphoid tissue.In the monkey #374,the peripheral CD4＋ T lymphocyte increased by 30% in the 6th month compared with the baseline level and then fluctuated.The plasma viral load gradually fell and reached about 1 log10 in the treatment group,but remained stable in the control group.As shown by pathological examinations,the lymph node and spleen of monkeys #374（treatment group） and #381（treatment group） showed rehabilitation and reconstruction in the lymphatic tissue,thymus,nerve tissue of gyrus hippocampi,pituitary gland,pineal body,thyroid gland,adrenal gland,and ovary.In the control group,however,animals experienced depletion of lymph nodes,atrophy of spleen,disappearance of thymus,and other disorders in endocrine organs.Conclusion Gonderma bucidum preparation may have certain protective effect on the immune system,nervous system,and endocrine system of monkeys with SAIDS.